Enzyme Production, Growth, and Adaptation of T. reesei Strains QM9414, L-27, RL-P37, and Rut C-30 to Conditioned Yellow Poplar Sawdust Hydrolysate: Scientific Note

Tammy Kay Hayward, Jenny Hamilton, David Templeton, Ed Jennings, Mark Ruth, Arun Tholudur, James D. McMillan, Mel Tucker, Ali Mohagheghi

Research output: Contribution to journalArticlepeer-review

12 Scopus Citations

Abstract

National Renewable Energy Laboratory (NREL) has developed a conditioning process that decreases acetic acid levels in pretreated yellow poplar hydrolysate. Trichoderma reesei is sensitive to acetic acid and this conditioning method has enabled applied cellulase production with hardwoods. T. reesei strains QM9414, L-27, RL-P37, and Rut C-30 were screened for growth on conditioned hydrolysate liquor. Tolerance to hydrolysate was found to be strain-dependent. Strain QM9414 was adapted tO grow in 80% (v/v) conditioned hydrolysate (40 g/L of soluble sugars and 1.6 g/L acetic acid from pretreated poplar). However, enzyme production was highest at 20% (v/v) hydrolysate using strain L-27. Cellulase titers of 2-3 International Filter Paper Units (IFPU)/mL were achieved using pretreated yellow poplar liquors and solids as the sole carbon sources.

Original languageAmerican English
Pages (from-to)293-309
Number of pages17
JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume77-79
DOIs
StatePublished - 1999

NREL Publication Number

  • NREL/JA-580-24739

Keywords

  • Acetic acid
  • Cellulase
  • Pretreated poplarhydrolysate
  • Trichoderma reesei furfural

Fingerprint

Dive into the research topics of 'Enzyme Production, Growth, and Adaptation of T. reesei Strains QM9414, L-27, RL-P37, and Rut C-30 to Conditioned Yellow Poplar Sawdust Hydrolysate: Scientific Note'. Together they form a unique fingerprint.

Cite this