Enzyme Scaffolds and Methods of Use

Yannick Bomble (Inventor), Michael Himmel (Inventor), Jeffrey Linger (Inventor), Roman Brunecky (Inventor), John Aikens (Inventor)

Research output: Patent


Polypeptide scaffolds comprising enzymatic proteins are provided. The enzymatic polypeptide scaffolds comprise heterologous enzymes to form a heterologous metabolic pathway, and can be targeted to a substrate through a surface anchoring domain. The enzymatic polypeptide scaffolds leverage the high specificity and affinity protein/protein interaction between the cohesins and dockerins of microorganismal cellulosomes to form custom enzymatic arrays
Original languageAmerican English
Patent number10,577,633 B2
Filing date3/03/20
StatePublished - 2020

NREL Publication Number

  • NREL/PT-2700-76406


  • dockerins
  • enzymatic proteins
  • heterologous enzymes
  • microorganismal cellulosomes
  • ohesins
  • polypeptide scaffolds


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