Evaluation of Spray-Drying Methods for Cellulase Preservation

M. Himmel, K. Oh, M. Tucker, C. Rivard, K. Grohmann

Research output: Contribution to conferencePaperpeer-review

5 Scopus Citations


Spray drying processes are widely used for the large-scale preservation of biological goods. The application of this technology to the enhancement of the storability of cellulases and related enzymes would improve fermentation processes requiring input of specific quantities of enzyme catalyst of known activity. The present study found that very high percentages (90% of original) of filter paper and carboxymethylcellulose hydrolyzing activities can be recovered from conventional spray dryers when operated at inlet air temperatures ranging from 90 to 180 degree C. The beta-glucosidase and beta-xylosidase activity recoveries were somewhat lower and were optimal at temperatures ranging from 120 to 150 degree C. However, the individual performance of the 12 enzyme preparations examined was quite preparation specific. An ultrasonic nozzle adaptation to the Yamato dryer demonstrated the overall feasibility of high energy atomization, inasmuch as the filter paper activity was retained after drying. The more labile beta-xylosidase activity was, however, entirely lost.

Original languageAmerican English
Number of pages11
StatePublished - 1986
EventEighth Symposium on Biotechnology for Fuels and Chemicals - Gatlinburg, Tennessee
Duration: 13 May 198616 May 1986


ConferenceEighth Symposium on Biotechnology for Fuels and Chemicals
CityGatlinburg, Tennessee

NREL Publication Number

  • ACNR/CP-232-8881


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