Evidence for a Dual Function of the Herbicide-Binding D1 Protein in Photosystem II

James G. Metz; Himadri B. Pakrasi; Michael Seibert; Charles J. Arntzer

doi:10.1016/0014-5793(86)80911-5

Evidence for a Dual Function of the Herbicide-Binding D1 Protein in Photosystem II

James G. Metz, Himadri B. Pakrasi, Michael Seibert, Charles J. Arntzer

BioEconomy and Sustainable Transportation

Research output: Contribution to journal › Article › peer-review

119 Scopus Citations

Abstract

The LF-1 mutant of the green alga Scenedesmus obliquus is completely blocked on the oxidizing (water-splitting) side of photosystem II (PS II) while the reaction center and reducing side remain functional. A 34-kDa protein found in the PS II reaction center core complex of wild-type cells is replaced by a 36-kDa protein in the mutant cells. Both of these proteins are labeled by azido[¹⁴C]atrazine and are recognized by polyclonal antibodies raised against the herbicide-binding, D1 protein of Amaranthus hybridus. The data provide a new perspective on the role of the D1 protein by implying that it affects the oxidizing side of PS II in addition to performing its well established function on the reducing side.

Original language	American English
Pages (from-to)	269-274
Number of pages	6
Journal	FEBS Letters
Volume	205
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(86)80911-5 https://doi.org/10.1016/0014-5793(86)80911-5
State	Published - 1986

Bibliographical note

Work performed by Photoconversion Research Branch, Solar Energy Research Institute, Golden, Colorado and Central Research & Development Department, E. I. DuPont de Nemours and Co., Wilmington, Delaware

NREL Publication Number

ACNR/JA-233-7965

Keywords

Azidoatrazine Herbicide binding Photosystem II Oxygen evolution (Scenedesmus
LF-1 mutant)

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title = "Evidence for a Dual Function of the Herbicide-Binding D1 Protein in Photosystem II",

abstract = "The LF-1 mutant of the green alga Scenedesmus obliquus is completely blocked on the oxidizing (water-splitting) side of photosystem II (PS II) while the reaction center and reducing side remain functional. A 34-kDa protein found in the PS II reaction center core complex of wild-type cells is replaced by a 36-kDa protein in the mutant cells. Both of these proteins are labeled by azido[14C]atrazine and are recognized by polyclonal antibodies raised against the herbicide-binding, D1 protein of Amaranthus hybridus. The data provide a new perspective on the role of the D1 protein by implying that it affects the oxidizing side of PS II in addition to performing its well established function on the reducing side.",

keywords = "Azidoatrazine Herbicide binding Photosystem II Oxygen evolution (Scenedesmus, LF-1 mutant)",

author = "Metz, {James G.} and Pakrasi, {Himadri B.} and Michael Seibert and Arntzer, {Charles J.}",

note = "Work performed by Photoconversion Research Branch, Solar Energy Research Institute, Golden, Colorado and Central Research & Development Department, E. I. DuPont de Nemours and Co., Wilmington, Delaware",

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doi = "10.1016/0014-5793(86)80911-5",

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AU - Metz, James G.

AU - Pakrasi, Himadri B.

AU - Seibert, Michael

AU - Arntzer, Charles J.

N1 - Work performed by Photoconversion Research Branch, Solar Energy Research Institute, Golden, Colorado and Central Research & Development Department, E. I. DuPont de Nemours and Co., Wilmington, Delaware

PY - 1986

Y1 - 1986

N2 - The LF-1 mutant of the green alga Scenedesmus obliquus is completely blocked on the oxidizing (water-splitting) side of photosystem II (PS II) while the reaction center and reducing side remain functional. A 34-kDa protein found in the PS II reaction center core complex of wild-type cells is replaced by a 36-kDa protein in the mutant cells. Both of these proteins are labeled by azido[14C]atrazine and are recognized by polyclonal antibodies raised against the herbicide-binding, D1 protein of Amaranthus hybridus. The data provide a new perspective on the role of the D1 protein by implying that it affects the oxidizing side of PS II in addition to performing its well established function on the reducing side.

AB - The LF-1 mutant of the green alga Scenedesmus obliquus is completely blocked on the oxidizing (water-splitting) side of photosystem II (PS II) while the reaction center and reducing side remain functional. A 34-kDa protein found in the PS II reaction center core complex of wild-type cells is replaced by a 36-kDa protein in the mutant cells. Both of these proteins are labeled by azido[14C]atrazine and are recognized by polyclonal antibodies raised against the herbicide-binding, D1 protein of Amaranthus hybridus. The data provide a new perspective on the role of the D1 protein by implying that it affects the oxidizing side of PS II in addition to performing its well established function on the reducing side.

KW - Azidoatrazine Herbicide binding Photosystem II Oxygen evolution (Scenedesmus

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DO - 10.1016/0014-5793(86)80911-5

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Evidence for a Dual Function of the Herbicide-Binding D1 Protein in Photosystem II

Abstract

Bibliographical note

NREL Publication Number

Keywords

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