@misc{18e3a164d5f14b638f37335fe260e09f,
title = "Expression and Characterization of Chimeric CBHI in Yeasts and the Effects of CBHI Fusion to EGII",
abstract = "The low secretion levels of cellobiohydrolaseI (CBHI) in yeasts is one of the key barriers preventing yeast from directly degrading and utilizing lignocellulose. To overcome this barrier, we have explored the approach of genetically linking an easily secreted protein to CBHI, with CBHI being the last to be folded. The Trichoderma reesei eg2 (TrEGII) gene was selected as the leading gene due to its previously demonstrated outstanding secretion in yeast. To comprehensively characterize the effects of this fusion protein, we tested this hypothesis in three industrially relevant yeasts: Saccharomyces cerevisiae, Yarrowia lipolytica, and Lipomyces starkeyi.",
keywords = "CBHI, cellobiohydrolaseI, Lipomyces starkeyi, Saccharomyces cerevisiae, Yarrowia lipolytica, yeasts",
author = "Petri Alahuhta and Qi Xu and Eric Knoshaug and Hui Wei and Wei Wang and Todd VanderWall and Michael Himmel and Min Zhang and Antonella Amore and John Baker",
year = "2018",
language = "American English",
series = "Presented at the 40th Symposium on Biotechnology for Fuels and Chemicals, 28 April - 2 May 2018, Clearwater, Florida",
type = "Other",
}