Expression and Characterization of Chimeric CBHI in Yeasts and the Effects of CBHI Fusion to EGII

Research output: NRELPoster

Abstract

The low secretion levels of cellobiohydrolaseI (CBHI) in yeasts is one of the key barriers preventing yeast from directly degrading and utilizing lignocellulose. To overcome this barrier, we have explored the approach of genetically linking an easily secreted protein to CBHI, with CBHI being the last to be folded. The Trichoderma reesei eg2 (TrEGII) gene was selected as the leading gene due to its previously demonstrated outstanding secretion in yeast. To comprehensively characterize the effects of this fusion protein, we tested this hypothesis in three industrially relevant yeasts: Saccharomyces cerevisiae, Yarrowia lipolytica, and Lipomyces starkeyi.
Original languageAmerican English
StatePublished - 2018

Publication series

NamePresented at the 40th Symposium on Biotechnology for Fuels and Chemicals, 28 April - 2 May 2018, Clearwater, Florida

NREL Publication Number

  • NREL/PO-2700-71427

Keywords

  • CBHI
  • cellobiohydrolaseI
  • Lipomyces starkeyi
  • Saccharomyces cerevisiae
  • Yarrowia lipolytica
  • yeasts

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