[FeFe]-Hydrogenases and Photobiological Hydrogen Production

Maria L. Ghirardi, Jordi Cohen, Paul King, Klaus Schulten, Kwiseon Kim, Michael Seibert

Research output: Contribution to conferencePaperpeer-review

20 Scopus Citations


The promise of efficient, economic and renewable H 2 photoproduction from water can potentially be met by green algae. These organisms are able to functionally link photosynthetic water oxidation to the catalytic recombination of protons and electrons to generate H 2 gas through the activity of the hydrogenase enzyme. Green algal hydrogenases contain a unique metallo-catalytic H-cluster that performs the reversible H 2 oxidation /evolution reactions. The H-cluster, located in the interior of the protein structure is irreversibly inactivated by O 2, the by-product of water oxidation. We developed an Escherichia coli expression system to produce [FeFe]-hydrogenases from different biological sources and demonstrated that clostridial [FeFe]-hydrogenases have higher tolerance to O 2 inactivation compared to their algal counterparts. We have been using computational simulations of gas diffusion within the Clostridium pasteurianum CpI hydrogenase to identify the pathways through which O 2 can reach its catalytic site. Subsequently, we modify the protein structure at specific sites along the O 2 pathways (identified by the computational simulations) by site-directed mutagenesis with the goal of generating recombinant enzymes with higher O 2 tolerance. In this paper, we review the computational simulation work and report on preliminary results obtained through this strategy.

Original languageAmerican English
Number of pages6
StatePublished - 2006
EventSolar Hydrogen and Nanotechnology - San Diego, CA, United States
Duration: 14 Aug 200617 Aug 2006


ConferenceSolar Hydrogen and Nanotechnology
Country/TerritoryUnited States
CitySan Diego, CA

NREL Publication Number

  • NREL/CP-270-44065


  • Clostridium
  • Computational simulation
  • Green algae
  • Hydrogen
  • Hydrogenase
  • O inactivation
  • Photosynthesis


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