Fingerprinting Trichoderma reesei Hydrolases in a Commercial Cellulase Preparation

T. B. Vinzant; W. S. Adney; S. R. Decker; J. O. Baker; M. T. Kinter; N. E. Sherman; J. W. Fox; M. E. Himmel

doi:10.1385/ABAB:91-93:1-9:99

Fingerprinting Trichoderma reesei Hydrolases in a Commercial Cellulase Preparation

T. B. Vinzant, W. S. Adney, S. R. Decker, J. O. Baker, M. T. Kinter, N. E. Sherman, J. W. Fox, M. E. Himmel

Research output: Contribution to journal › Article › peer-review

105 Scopus Citations

Abstract

Polysaccharide degrading enzymes from commercial T. reesei broth have been subjected to "fingerprint" analysis by high-resolution 2-D gel electrophoresis. Forty-five spots from 11 × 25 cm Pharmacia gels have been analyzed by LC-MS/MS and the resulting peptide sequences were compared to existing databases. Understanding the roles and relationships of component enzymes from the T. reesei cellulase system acting on complex substrates is key to the development of efficient artificial cellulase systems for the conversion of lignocellulosic biomass to sugars. These studies suggest follow-on work comparing induced and noninduced T. reesei cells at the proteome level, which may elucidate substrate-specific gene regulation and response.

Original language	American English
Pages (from-to)	99-107
Number of pages	9
Journal	Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume	91-93
DOIs	https://doi.org/10.1385/ABAB:91-93:1-9:99 https://doi.org/10.1385/ABAB:91-93:1-9:99
State	Published - 2001

NREL Publication Number

NREL/JA-580-30578

Keywords

Cellulase
Liquid chromatography-mass spectrometry/mass spectrometry
Trichoderma reesei
Two-dimensional gel electrophoresis

Access to Document

Cite this

Vinzant, T. B., Adney, W. S., Decker, S. R., Baker, J. O., Kinter, M. T., Sherman, N. E., Fox, J. W., & Himmel, M. E. (2001). Fingerprinting Trichoderma reesei Hydrolases in a Commercial Cellulase Preparation. Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology, 91-93, 99-107. https://doi.org/10.1385/ABAB:91-93:1-9:99, https://doi.org/10.1385/ABAB:91-93:1-9:99

@article{57482595847d40b28e89600c40f07395,

title = "Fingerprinting Trichoderma reesei Hydrolases in a Commercial Cellulase Preparation",

abstract = "Polysaccharide degrading enzymes from commercial T. reesei broth have been subjected to {"}fingerprint{"} analysis by high-resolution 2-D gel electrophoresis. Forty-five spots from 11 × 25 cm Pharmacia gels have been analyzed by LC-MS/MS and the resulting peptide sequences were compared to existing databases. Understanding the roles and relationships of component enzymes from the T. reesei cellulase system acting on complex substrates is key to the development of efficient artificial cellulase systems for the conversion of lignocellulosic biomass to sugars. These studies suggest follow-on work comparing induced and noninduced T. reesei cells at the proteome level, which may elucidate substrate-specific gene regulation and response.",

keywords = "Cellulase, Liquid chromatography-mass spectrometry/mass spectrometry, Trichoderma reesei, Two-dimensional gel electrophoresis",

author = "Vinzant, {T. B.} and Adney, {W. S.} and Decker, {S. R.} and Baker, {J. O.} and Kinter, {M. T.} and Sherman, {N. E.} and Fox, {J. W.} and Himmel, {M. E.}",

year = "2001",

doi = "10.1385/ABAB:91-93:1-9:99",

language = "American English",

volume = "91-93",

pages = "99--107",

journal = "Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology",

issn = "0273-2289",

publisher = "Springer",

}

Vinzant, TB, Adney, WS, Decker, SR, Baker, JO, Kinter, MT, Sherman, NE, Fox, JW & Himmel, ME 2001, 'Fingerprinting Trichoderma reesei Hydrolases in a Commercial Cellulase Preparation', Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology, vol. 91-93, pp. 99-107. https://doi.org/10.1385/ABAB:91-93:1-9:99, https://doi.org/10.1385/ABAB:91-93:1-9:99

TY - JOUR

T1 - Fingerprinting Trichoderma reesei Hydrolases in a Commercial Cellulase Preparation

AU - Vinzant, T. B.

AU - Adney, W. S.

AU - Decker, S. R.

AU - Baker, J. O.

AU - Kinter, M. T.

AU - Sherman, N. E.

AU - Fox, J. W.

AU - Himmel, M. E.

PY - 2001

Y1 - 2001

N2 - Polysaccharide degrading enzymes from commercial T. reesei broth have been subjected to "fingerprint" analysis by high-resolution 2-D gel electrophoresis. Forty-five spots from 11 × 25 cm Pharmacia gels have been analyzed by LC-MS/MS and the resulting peptide sequences were compared to existing databases. Understanding the roles and relationships of component enzymes from the T. reesei cellulase system acting on complex substrates is key to the development of efficient artificial cellulase systems for the conversion of lignocellulosic biomass to sugars. These studies suggest follow-on work comparing induced and noninduced T. reesei cells at the proteome level, which may elucidate substrate-specific gene regulation and response.

AB - Polysaccharide degrading enzymes from commercial T. reesei broth have been subjected to "fingerprint" analysis by high-resolution 2-D gel electrophoresis. Forty-five spots from 11 × 25 cm Pharmacia gels have been analyzed by LC-MS/MS and the resulting peptide sequences were compared to existing databases. Understanding the roles and relationships of component enzymes from the T. reesei cellulase system acting on complex substrates is key to the development of efficient artificial cellulase systems for the conversion of lignocellulosic biomass to sugars. These studies suggest follow-on work comparing induced and noninduced T. reesei cells at the proteome level, which may elucidate substrate-specific gene regulation and response.

KW - Cellulase

KW - Liquid chromatography-mass spectrometry/mass spectrometry

KW - Trichoderma reesei

KW - Two-dimensional gel electrophoresis

UR - http://www.scopus.com/inward/record.url?scp=0035030366&partnerID=8YFLogxK

U2 - 10.1385/ABAB:91-93:1-9:99

DO - 10.1385/ABAB:91-93:1-9:99

M3 - Article

C2 - 11963908

AN - SCOPUS:0035030366

SN - 0273-2289

VL - 91-93

SP - 99

EP - 107

JO - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

JF - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

ER -

Fingerprinting Trichoderma reesei Hydrolases in a Commercial Cellulase Preparation

Abstract

NREL Publication Number

Keywords

Access to Document

Other files and links

Fingerprint

Cite this