Fingerprinting Trichoderma reesei Hydrolases in a Commercial Cellulase Preparation

T. B. Vinzant, W. S. Adney, S. R. Decker, J. O. Baker, M. T. Kinter, N. E. Sherman, J. W. Fox, M. E. Himmel

Research output: Contribution to journalArticlepeer-review

105 Scopus Citations


Polysaccharide degrading enzymes from commercial T. reesei broth have been subjected to "fingerprint" analysis by high-resolution 2-D gel electrophoresis. Forty-five spots from 11 × 25 cm Pharmacia gels have been analyzed by LC-MS/MS and the resulting peptide sequences were compared to existing databases. Understanding the roles and relationships of component enzymes from the T. reesei cellulase system acting on complex substrates is key to the development of efficient artificial cellulase systems for the conversion of lignocellulosic biomass to sugars. These studies suggest follow-on work comparing induced and noninduced T. reesei cells at the proteome level, which may elucidate substrate-specific gene regulation and response.

Original languageAmerican English
Pages (from-to)99-107
Number of pages9
JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
StatePublished - 2001

NREL Publication Number

  • NREL/JA-580-30578


  • Cellulase
  • Liquid chromatography-mass spectrometry/mass spectrometry
  • Trichoderma reesei
  • Two-dimensional gel electrophoresis


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