Fluorescence Imaging In Vivo at Wavelengths Beyond 1500 nm

Shuo Diao, Jeffrey L. Blackburn, Guosong Hong, Alexander L. Antaris, Junlei Chang, Justin Z. Wu, Bo Zhang, Kai Cheng, Calvin J. Kuo, Hongjie Dai

Research output: Contribution to journalArticlepeer-review

313 Scopus Citations

Abstract

Compared to imaging in the visible and near-infrared regions below 900 nm, imaging in the second near-infrared window (NIR-II, 1000-1700 nm) is a promising method for deep-tissue high-resolution optical imaging in vivo mainly owing to the reduced scattering of photons traversing through biological tissues. Herein, semiconducting single-walled carbon nanotubes with large diameters were used for in vivo fluorescence imaging in the long-wavelength NIR region (1500-1700 nm, NIR-IIb). With this imaging agent, 3-4 μm wide capillary blood vessels at a depth of about 3 mm could be resolved. Meanwhile, the blood-flow speeds in multiple individual vessels could be mapped simultaneously. Furthermore, NIR-IIb tumor imaging of a live mouse was explored. NIR-IIb imaging can be generalized to a wide range of fluorophores emitting at up to 1700 nm for high-performance in vivo optical imaging. Semiconducting single-walled carbon nanotubes with large diameters were used for in vivo fluorescence imaging in the long-wavelength near-infrared region (1500-1700 nm). With this imaging agent, 3-4 μm wide capillary blood vessels at a depth of about 3 mm in living mice could be resolved, and the blood-flow speeds in multiple individual vessels were mapped simultaneously.

Original languageAmerican English
Pages (from-to)14758-14762
Number of pages5
JournalAngewandte Chemie - International Edition
Volume54
Issue number49
DOIs
StatePublished - 2015

Bibliographical note

Publisher Copyright:
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

NREL Publication Number

  • NREL/JA-5900-61423

Keywords

  • carbon nanotubes
  • fluorescence
  • imaging
  • in vivo

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