Genome-Wide Association Study Reveals Putative Regulators of Bioenergy Traits in Populus deltoides

Annette M. Fahrenkrog, Leandro G. Neves, Márcio F.R. Resende, Ana I. Vazquez, Gustavo de los Campos, Christopher Dervinis, Robert Sykes, Mark Davis, Ruth Davenport, William B. Barbazuk, Matias Kirst

Research output: Contribution to journalArticlepeer-review

74 Scopus Citations

Abstract

Genome-wide association studies (GWAS) have been used extensively to dissect the genetic regulation of complex traits in plants. These studies have focused largely on the analysis of common genetic variants despite the abundance of rare polymorphisms in several species, and their potential role in trait variation. Here, we conducted the first GWAS in Populus deltoides, a genetically diverse keystone forest species in North America and an important short rotation woody crop for the bioenergy industry. We searched for associations between eight growth and wood composition traits, and common and low-frequency single-nucleotide polymorphisms detected by targeted resequencing of 18 153 genes in a population of 391 unrelated individuals. To increase power to detect associations with low-frequency variants, multiple-marker association tests were used in combination with single-marker association tests. Significant associations were discovered for all phenotypes and are indicative that low-frequency polymorphisms contribute to phenotypic variance of several bioenergy traits. Our results suggest that both common and low-frequency variants need to be considered for a comprehensive understanding of the genetic regulation of complex traits, particularly in species that carry large numbers of rare polymorphisms. These polymorphisms may be critical for the development of specialized plant feedstocks for bioenergy.

Original languageAmerican English
Pages (from-to)799-811
Number of pages13
JournalNew Phytologist
Volume213
Issue number2
DOIs
StatePublished - 2017

Bibliographical note

Publisher Copyright:
© 2016 The Authors. New Phytologist © 2016 New Phytologist Trust

NREL Publication Number

  • NREL/JA-5100-67133

Keywords

  • eastern cottonwood
  • exome resequencing
  • genome-wide association studies (GWAS)
  • low-frequency single-nucleotide polymorphisms (SNPs)
  • poplar
  • Populus deltoides

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