Harnessing Glycosylation to Improve Cellulase Activity

Gregg T. Beckham, Ziyu Dai, James F. Matthews, Michelle Momany, Christina M. Payne, William S. Adney, Scott E. Baker, Michael E. Himmel

Research output: Contribution to journalArticlepeer-review

104 Scopus Citations

Abstract

Cellulases and hemicellulases are responsible for the turnover of plant cell wall polysaccharides in the biosphere, and thus form the foundation of enzyme engineering efforts in biofuels research. Many of these carbohydrate-active enzymes from filamentous fungi contain both N-linked and O-linked glycosylation, the extent and heterogeneity of which depends on growth conditions, expression host, and the presence of glycan trimming enzymes in the secretome, all of which in turn impact enzyme activity. As the roles of glycosylation in enzyme function have not been fully elucidated, here we discuss the potential roles of glycosylation on glycoside hydrolase enzyme structure and function after secretion. We posit that glycosylation, instead of hindering cellulase engineering, can be used as an additional tool to enhance enzyme activity, given deeper understanding of its molecular-level role in biomass deconstruction.

Original languageAmerican English
Pages (from-to)338-345
Number of pages8
JournalCurrent Opinion in Biotechnology
Volume23
Issue number3
DOIs
StatePublished - 2012

NREL Publication Number

  • NREL/JA-5100-53669

Keywords

  • biofuels
  • cellulases
  • enzyme activity
  • glycosylation
  • hemicellulases

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