Heterologous Protein Expression in Hypocrea jecorina: A Historical Perspective and New Developments

Arjun Singh, Larry E. Taylor, Todd A. Vander Wall, Jeffrey Linger, Michael E. Himmel, Kara Podkaminer, William S. Adney, Stephen R. Decker

Research output: Contribution to journalArticlepeer-review

47 Scopus Citations

Abstract

Hypocrea jecorina, the sexual teleomorph of Trichoderma reesei, has long been favored as an industrial cellulase producer, first utilizing its native cellulase system and later augmented by the introduction of heterologous enzymatic activities or improved variants of native enzymes. Expression of heterologous proteins in H. jecorina was once considered difficult when the target was an improved variant of a native cellulase. Developments over the past nearly 30. years have produced strains, vectors, and selection mechanisms that have continued to simplify and streamline heterologous protein expression in this fungus. More recent developments in fungal molecular biology have pointed the way toward a fundamental transformation in the ease and efficiency of heterologous protein expression in this important industrial host. Here, 1) we provide a historical perspective on advances in H. jecorina molecular biology, 2) outline host strain engineering, transformation, selection, and expression strategies, 3) detail potential pitfalls when working with this organism, and 4) provide consolidated examples of successful cellulase expression outcomes from our laboratory.

Original languageAmerican English
Pages (from-to)142-154
Number of pages13
JournalBiotechnology Advances
Volume33
Issue number1
DOIs
StatePublished - 2015

Bibliographical note

Publisher Copyright:
© 2014.

NREL Publication Number

  • NREL/JA-2700-62546

Keywords

  • Cbh1
  • Cbh1 deletion
  • Cel7A
  • Cellobiohydrolase
  • Cellulase expression systems
  • Fungal transformation
  • Hypocrea jecorina
  • QM6a
  • Rut-C30
  • Trichoderma reesei

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