High-Throughput Functional Genomics for Energy Production

Jacob Fenster, Carrie Eckert

Research output: Contribution to journalArticlepeer-review

7 Scopus Citations

Abstract

Functional genomics remains a foundational field for establishing genotype-phenotype relationships that enable strain engineering. High-throughput (HTP) methods accelerate the Design-Build-Test-Learn cycle that currently drives synthetic biology towards a forward engineering future. Trackable mutagenesis techniques including transposon insertion sequencing and CRISPR-Cas-mediated genome editing allow for rapid fitness profiling of a collection, or library, of mutants to discover beneficial mutations. Due to the relative speed of these experiments compared to adaptive evolution experiments, iterative rounds of mutagenesis can be implemented for next-generation metabolic engineering efforts to design complex production and tolerance phenotypes. Additionally, the expansion of these mutagenesis techniques to novel bacteria are opening up industrial microbes that show promise for establishing a bio-based economy.
Original languageAmerican English
Pages (from-to)7-14
Number of pages8
JournalCurrent Opinion in Biotechnology
Volume67
DOIs
StatePublished - 2021

NREL Publication Number

  • NREL/JA-2700-77739

Keywords

  • genomic methods
  • high-throughput
  • microbial systems

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