High-Yield Shake-Flask Fermentation of Xylose to Ethanol

Brian L. Boynton, James D. McMillan

Research output: Contribution to journalArticlepeer-review

10 Scopus Citations


Methods of achieving high-yield fermentation of d-xylose to ethanol in shake flasks were investigated using the microaerophilic yeast Pichia stipitis NRRL Y-7124. Ethanol yields based on consumed xylose approached the theoretical maximum of 0.51 g/g at high culture volumes. Although volumetric productivities decreased from 0.46 g/L-h at 100 mL to 0.15 g/L-h at 225 mL, ethanol yields on consumed xylose of 0.50 g/g were achieved in 250-mL DeLong culture flasks at culture volumes of 200 and 225 mL.

Original languageAmerican English
Pages (from-to)509-514
Number of pages6
JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Issue number1
StatePublished - 1994

NREL Publication Number

  • NREL/JA-423-5471


  • d-xylose
  • ethanol
  • fermentation
  • Pichia stipitis
  • shake flask


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