Highly Efficient Libraries Design for Saturation Mutagenesis: Article No. ysac006

Gur Pines, Assaf Pines, Carrie Eckert

Research output: Contribution to journalArticlepeer-review

2 Scopus Citations

Abstract

Saturation mutagenesis is a semi-rational approach for protein engineering where sites are saturated either entirely or partially to include amino acids of interest. We previously reported on a codon compression algorithm, where a set of minimal degenerate codons are selected according to user-defined parameters such as the target organism, type of saturation and usage levels. Here, we communicate an addition to our web tool that considers the distance between the wild-type codon and the library, depending on its purpose. These forms of restricted collections further reduce library size, lowering downstream screening efforts or, in turn, allowing more comprehensive saturation of multiple sites. The library design tool can be accessed via http://www.dynamcc.com/dynamcc_d/.
Original languageAmerican English
Number of pages6
JournalSynthetic Biology
Volume7
Issue number1
DOIs
StatePublished - 2022

NREL Publication Number

  • NREL/JA-2700-78936

Keywords

  • design software
  • mutagenesis
  • protein engineering

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