How Does Plant Cell Wall Nanoscale Architecture Correlate with Enzymatic Digestibility?

Shi You Ding, Yu San Liu, Yining Zeng, Michael E. Himmel, John O. Baker, Edward A. Bayer

Research output: Contribution to journalArticlepeer-review

635 Scopus Citations

Abstract

Greater understanding of the mechanisms contributing to chemical and enzymatic solubilization of plant cell walls is critical for enabling cost-effective industrial conversion of cellulosic biomass to biofuels. Here, we report the use of correlative imaging in real time to assess the impact of pretreatment, as well as the resulting nanometer-scale changes in cell wall structure, upon subsequent digestion by two commercially relevant cellulase systems. We demonstrate that the small, noncomplexed fungal cellulases deconstruct cell walls using mechanisms that differ considerably from those of the larger, multienzyme complexes (cellulosomes). Furthermore, high-resolution measurement of the microfibrillar architecture of cell walls suggests that digestion is primarily facilitated by enabling enzyme access to the hydrophobic cellulose face. The data support the conclusion that ideal pretreatments should maximize lignin removal and minimize polysaccharide modification, thereby retaining the essentially native microfibrillar structure.

Original languageAmerican English
Pages (from-to)1055-1060
Number of pages6
JournalScience
Volume338
Issue number6110
DOIs
StatePublished - 23 Nov 2012

NREL Publication Number

  • NREL/JA-2700-56061

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