Hydration and saccharification of cellulose Iβ, II and IIII at increasing dry solids loadings

Michael J. Selig; Lisbeth G. Thygesen; David K. Johnson; Michael E. Himmel; Claus Felby; Ashutosh Mittal

doi:10.1007/s10529-013-1258-7

Hydration and saccharification of cellulose Iβ, II and III_I at increasing dry solids loadings

Michael J. Selig, Lisbeth G. Thygesen, David K. Johnson, Michael E. Himmel, Claus Felby, Ashutosh Mittal

University of Copenhagen

Research output: Contribution to journal › Article › peer-review

21 Scopus Citations

Abstract

Crystalline cellulose Iβ (Avicel) was chemically transformed into cellulose II and III_I producing allomorphs with similar crystallinity indices (ATR-IR and XRD derived). Saccharifications by commercial cellulases at arrayed solids loadings showed cellulose III_I was more readily hydrolysable and less susceptible to increased dry solids levels than cellulose Iβ and II. Analysis by dynamic vapor sorption revealed cellulose II has a distinctively higher absorptive capacity than cellulose I and III_I. When equally hydrated (g water/g cellulose), low-field nuclear magnetic resonance (LF-NMR) relaxometry showed that cellulose II, on average, most constrained water while cellulase III_I left the most free water. LF-NMR spin-spin relaxation time distribution profiles representing distinct water pools suggest cellulose III_I had the most restricted pool and changes in water distribution during enzymatic saccharification were most dramatic with respect to cellulose III_I compared to celluloses Iβ and II.

Original language	American English
Pages (from-to)	1599-1607
Number of pages	9
Journal	Biotechnology Letters
Volume	35
Issue number	10
DOIs	https://doi.org/10.1007/s10529-013-1258-7 https://doi.org/10.1007/s10529-013-1258-7
State	Published - 2013

NREL Publication Number

NREL/JA-2700-60769

Keywords

Cellulose
Crystalline allomorph
High solids
Hydration
Saccharification

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Cite this

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title = "Hydration and saccharification of cellulose Iβ, II and IIII at increasing dry solids loadings",

abstract = "Crystalline cellulose Iβ (Avicel) was chemically transformed into cellulose II and IIII producing allomorphs with similar crystallinity indices (ATR-IR and XRD derived). Saccharifications by commercial cellulases at arrayed solids loadings showed cellulose IIII was more readily hydrolysable and less susceptible to increased dry solids levels than cellulose Iβ and II. Analysis by dynamic vapor sorption revealed cellulose II has a distinctively higher absorptive capacity than cellulose I and IIII. When equally hydrated (g water/g cellulose), low-field nuclear magnetic resonance (LF-NMR) relaxometry showed that cellulose II, on average, most constrained water while cellulase IIII left the most free water. LF-NMR spin-spin relaxation time distribution profiles representing distinct water pools suggest cellulose IIII had the most restricted pool and changes in water distribution during enzymatic saccharification were most dramatic with respect to cellulose IIII compared to celluloses Iβ and II.",

keywords = "Cellulose, Crystalline allomorph, High solids, Hydration, Saccharification",

author = "Selig, {Michael J.} and Thygesen, {Lisbeth G.} and Johnson, {David K.} and Himmel, {Michael E.} and Claus Felby and Ashutosh Mittal",

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T1 - Hydration and saccharification of cellulose Iβ, II and IIII at increasing dry solids loadings

AU - Selig, Michael J.

AU - Thygesen, Lisbeth G.

AU - Johnson, David K.

AU - Himmel, Michael E.

AU - Felby, Claus

AU - Mittal, Ashutosh

PY - 2013

Y1 - 2013

N2 - Crystalline cellulose Iβ (Avicel) was chemically transformed into cellulose II and IIII producing allomorphs with similar crystallinity indices (ATR-IR and XRD derived). Saccharifications by commercial cellulases at arrayed solids loadings showed cellulose IIII was more readily hydrolysable and less susceptible to increased dry solids levels than cellulose Iβ and II. Analysis by dynamic vapor sorption revealed cellulose II has a distinctively higher absorptive capacity than cellulose I and IIII. When equally hydrated (g water/g cellulose), low-field nuclear magnetic resonance (LF-NMR) relaxometry showed that cellulose II, on average, most constrained water while cellulase IIII left the most free water. LF-NMR spin-spin relaxation time distribution profiles representing distinct water pools suggest cellulose IIII had the most restricted pool and changes in water distribution during enzymatic saccharification were most dramatic with respect to cellulose IIII compared to celluloses Iβ and II.

AB - Crystalline cellulose Iβ (Avicel) was chemically transformed into cellulose II and IIII producing allomorphs with similar crystallinity indices (ATR-IR and XRD derived). Saccharifications by commercial cellulases at arrayed solids loadings showed cellulose IIII was more readily hydrolysable and less susceptible to increased dry solids levels than cellulose Iβ and II. Analysis by dynamic vapor sorption revealed cellulose II has a distinctively higher absorptive capacity than cellulose I and IIII. When equally hydrated (g water/g cellulose), low-field nuclear magnetic resonance (LF-NMR) relaxometry showed that cellulose II, on average, most constrained water while cellulase IIII left the most free water. LF-NMR spin-spin relaxation time distribution profiles representing distinct water pools suggest cellulose IIII had the most restricted pool and changes in water distribution during enzymatic saccharification were most dramatic with respect to cellulose IIII compared to celluloses Iβ and II.

KW - Cellulose

KW - Crystalline allomorph

KW - High solids

KW - Hydration

KW - Saccharification

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JF - Biotechnology Letters

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