Hydrogen Production from a Carbon-Monoxide Oxidation Pathway in Rubrivivax gelatinosus

Pin Ching Maness; Paul F. Weaver

doi:10.1016/S0360-3199(02)00107-6

Hydrogen Production from a Carbon-Monoxide Oxidation Pathway in Rubrivivax gelatinosus

Pin Ching Maness, Paul F. Weaver

Bioenergy Science and Technology

National Renewable Energy Laboratory

Research output: Contribution to journal › Article › peer-review

42 Scopus Citations

Abstract

Upon feeding CO to the gas phase of a photosynthetic bacterium Rubrivivax gelatinosus CBS, a CO oxidation: H₂ production pathway is quickly induced. Hydrogen is produced according to the equation CO + H₂O → CO₂ + H₂. Two enzymes are known to be involved in this pathway: a CO dehydrogenase (CODH) with a pH optimum of 8.0 and above, and a hydrogenase with a pH optimum near 7.5. Carbon monoxide dehydrogenase also displays a temperature optimum near 50°C. When CO mass transfer is not limited during a CO uptake measurement, an extreme fast rate of CO uptake was determined, allowing for the removal of near 87% of the dissolved CO from a bacterial suspension within 10 s. This process has therefore two potential applications, one in the production of H₂ gas as a clean renewable fuel using the linked CO oxidation: H₂ production pathway, and another in using the CODH enzyme itself as a fuel-gas conditioning catalyst. These applications thereby will improve the overall H₂ economy when gasified waste biomass serves as the inexpensive feedstock.

Original language	American English
Pages (from-to)	1407-1411
Number of pages	5
Journal	International Journal of Hydrogen Energy
Volume	27
Issue number	11-12
DOIs	https://doi.org/10.1016/S0360-3199(02)00107-6 https://doi.org/10.1016/S0360-3199(02)00107-6
State	Published - 2002

NREL Publication Number

NREL/JA-590-33326

Keywords

Carbon monoxide
Carbon monoxide dehydrogenase
Hydrogen
Hydrogenase
Rubrivivax gelatinosus

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title = "Hydrogen Production from a Carbon-Monoxide Oxidation Pathway in Rubrivivax gelatinosus",

abstract = "Upon feeding CO to the gas phase of a photosynthetic bacterium Rubrivivax gelatinosus CBS, a CO oxidation: H2 production pathway is quickly induced. Hydrogen is produced according to the equation CO + H2O → CO2 + H2. Two enzymes are known to be involved in this pathway: a CO dehydrogenase (CODH) with a pH optimum of 8.0 and above, and a hydrogenase with a pH optimum near 7.5. Carbon monoxide dehydrogenase also displays a temperature optimum near 50°C. When CO mass transfer is not limited during a CO uptake measurement, an extreme fast rate of CO uptake was determined, allowing for the removal of near 87% of the dissolved CO from a bacterial suspension within 10 s. This process has therefore two potential applications, one in the production of H2 gas as a clean renewable fuel using the linked CO oxidation: H2 production pathway, and another in using the CODH enzyme itself as a fuel-gas conditioning catalyst. These applications thereby will improve the overall H2 economy when gasified waste biomass serves as the inexpensive feedstock.",

keywords = "Carbon monoxide, Carbon monoxide dehydrogenase, Hydrogen, Hydrogenase, Rubrivivax gelatinosus",

author = "Maness, {Pin Ching} and Weaver, {Paul F.}",

year = "2002",

doi = "10.1016/S0360-3199(02)00107-6",

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pages = "1407--1411",

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TY - JOUR

T1 - Hydrogen Production from a Carbon-Monoxide Oxidation Pathway in Rubrivivax gelatinosus

AU - Maness, Pin Ching

AU - Weaver, Paul F.

PY - 2002

Y1 - 2002

N2 - Upon feeding CO to the gas phase of a photosynthetic bacterium Rubrivivax gelatinosus CBS, a CO oxidation: H2 production pathway is quickly induced. Hydrogen is produced according to the equation CO + H2O → CO2 + H2. Two enzymes are known to be involved in this pathway: a CO dehydrogenase (CODH) with a pH optimum of 8.0 and above, and a hydrogenase with a pH optimum near 7.5. Carbon monoxide dehydrogenase also displays a temperature optimum near 50°C. When CO mass transfer is not limited during a CO uptake measurement, an extreme fast rate of CO uptake was determined, allowing for the removal of near 87% of the dissolved CO from a bacterial suspension within 10 s. This process has therefore two potential applications, one in the production of H2 gas as a clean renewable fuel using the linked CO oxidation: H2 production pathway, and another in using the CODH enzyme itself as a fuel-gas conditioning catalyst. These applications thereby will improve the overall H2 economy when gasified waste biomass serves as the inexpensive feedstock.

AB - Upon feeding CO to the gas phase of a photosynthetic bacterium Rubrivivax gelatinosus CBS, a CO oxidation: H2 production pathway is quickly induced. Hydrogen is produced according to the equation CO + H2O → CO2 + H2. Two enzymes are known to be involved in this pathway: a CO dehydrogenase (CODH) with a pH optimum of 8.0 and above, and a hydrogenase with a pH optimum near 7.5. Carbon monoxide dehydrogenase also displays a temperature optimum near 50°C. When CO mass transfer is not limited during a CO uptake measurement, an extreme fast rate of CO uptake was determined, allowing for the removal of near 87% of the dissolved CO from a bacterial suspension within 10 s. This process has therefore two potential applications, one in the production of H2 gas as a clean renewable fuel using the linked CO oxidation: H2 production pathway, and another in using the CODH enzyme itself as a fuel-gas conditioning catalyst. These applications thereby will improve the overall H2 economy when gasified waste biomass serves as the inexpensive feedstock.

KW - Carbon monoxide

KW - Carbon monoxide dehydrogenase

KW - Hydrogen

KW - Hydrogenase

KW - Rubrivivax gelatinosus

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U2 - 10.1016/S0360-3199(02)00107-6

DO - 10.1016/S0360-3199(02)00107-6

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SN - 0360-3199

VL - 27

SP - 1407

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JO - International Journal of Hydrogen Energy

JF - International Journal of Hydrogen Energy

IS - 11-12

ER -

Hydrogen Production from a Carbon-Monoxide Oxidation Pathway in Rubrivivax gelatinosus

Abstract

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Keywords

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