Abstract
The Laboratory Analytical Procedure (LAP) outlined here describes a method to quantitatively extract phytopigments from microalgae biomass, as well as to identify and quantify individual pigments based on separation and detection with a High-Performance Liquid Chromatography (HPLC) system coupled to a Diode Array Detector (DAD). Pigments were extracted with greater than 95 % extraction efficiency. Chromatographic conditions allowed for isomeric resolution between pigments and identification based on UV/Vis spectra and comparison to analytical standards.
Original language | American English |
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Number of pages | 28 |
DOIs | |
State | Published - 2022 |
NREL Publication Number
- NREL/TP-5100-84170
Keywords
- algae
- carotenoid
- chemical composition
- chlorophyll
- lipids
- pigments
- xanthophyll