Identification and Quantification of Photosynthetic Pigments in Algae. Laboratory Analytical Procedure (LAP), Issue Date: October 27, 2022

Research output: NRELTechnical Report

Abstract

The Laboratory Analytical Procedure (LAP) outlined here describes a method to quantitatively extract phytopigments from microalgae biomass, as well as to identify and quantify individual pigments based on separation and detection with a High-Performance Liquid Chromatography (HPLC) system coupled to a Diode Array Detector (DAD). Pigments were extracted with greater than 95 % extraction efficiency. Chromatographic conditions allowed for isomeric resolution between pigments and identification based on UV/Vis spectra and comparison to analytical standards.
Original languageAmerican English
Number of pages28
DOIs
StatePublished - 2022

NREL Publication Number

  • NREL/TP-5100-84170

Keywords

  • algae
  • carotenoid
  • chemical composition
  • chlorophyll
  • lipids
  • pigments
  • xanthophyll

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