Identification and Quantification of Photosynthetic Pigments in Algae. Laboratory Analytical Procedure (LAP), Issue Date: October 27, 2022

Steven Rowland; Stefanie Van Wychen; Lieve Laurens

doi:10.2172/1896813

Identification and Quantification of Photosynthetic Pigments in Algae. Laboratory Analytical Procedure (LAP), Issue Date: October 27, 2022

Research output: NLR › Technical Report

Abstract

The Laboratory Analytical Procedure (LAP) outlined here describes a method to quantitatively extract phytopigments from microalgae biomass, as well as to identify and quantify individual pigments based on separation and detection with a High-Performance Liquid Chromatography (HPLC) system coupled to a Diode Array Detector (DAD). Pigments were extracted with greater than 95 % extraction efficiency. Chromatographic conditions allowed for isomeric resolution between pigments and identification based on UV/Vis spectra and comparison to analytical standards.

Original language	American English
Number of pages	28
DOIs	https://doi.org/10.2172/1896813
State	Published - 2022

NLR Publication Number

NREL/TP-5100-84170

Keywords

algae
carotenoid
chemical composition
chlorophyll
lipids
pigments
xanthophyll

Access to Document

10.2172/1896813

https://www.nrel.gov/docs/fy23osti/84170.pdf

Cite this

@misc{72fc5cb385b943c9a85abafada0e4870,

title = "Identification and Quantification of Photosynthetic Pigments in Algae. Laboratory Analytical Procedure (LAP), Issue Date: October 27, 2022",

abstract = "The Laboratory Analytical Procedure (LAP) outlined here describes a method to quantitatively extract phytopigments from microalgae biomass, as well as to identify and quantify individual pigments based on separation and detection with a High-Performance Liquid Chromatography (HPLC) system coupled to a Diode Array Detector (DAD). Pigments were extracted with greater than 95 \% extraction efficiency. Chromatographic conditions allowed for isomeric resolution between pigments and identification based on UV/Vis spectra and comparison to analytical standards.",

keywords = "algae, carotenoid, chemical composition, chlorophyll, lipids, pigments, xanthophyll",

author = "Steven Rowland and \{Van Wychen\}, Stefanie and Lieve Laurens",

year = "2022",

doi = "10.2172/1896813",

language = "American English",

type = "Other",

}

TY - GEN

T1 - Identification and Quantification of Photosynthetic Pigments in Algae. Laboratory Analytical Procedure (LAP), Issue Date: October 27, 2022

AU - Rowland, Steven

AU - Van Wychen, Stefanie

AU - Laurens, Lieve

PY - 2022

Y1 - 2022

N2 - The Laboratory Analytical Procedure (LAP) outlined here describes a method to quantitatively extract phytopigments from microalgae biomass, as well as to identify and quantify individual pigments based on separation and detection with a High-Performance Liquid Chromatography (HPLC) system coupled to a Diode Array Detector (DAD). Pigments were extracted with greater than 95 % extraction efficiency. Chromatographic conditions allowed for isomeric resolution between pigments and identification based on UV/Vis spectra and comparison to analytical standards.

AB - The Laboratory Analytical Procedure (LAP) outlined here describes a method to quantitatively extract phytopigments from microalgae biomass, as well as to identify and quantify individual pigments based on separation and detection with a High-Performance Liquid Chromatography (HPLC) system coupled to a Diode Array Detector (DAD). Pigments were extracted with greater than 95 % extraction efficiency. Chromatographic conditions allowed for isomeric resolution between pigments and identification based on UV/Vis spectra and comparison to analytical standards.

KW - algae

KW - carotenoid

KW - chemical composition

KW - chlorophyll

KW - lipids

KW - pigments

KW - xanthophyll

U2 - 10.2172/1896813

DO - 10.2172/1896813

M3 - Technical Report

ER -

Identification and Quantification of Photosynthetic Pigments in Algae. Laboratory Analytical Procedure (LAP), Issue Date: October 27, 2022

Abstract

NLR Publication Number

Keywords

Access to Document

Fingerprint

Cite this