Identification of Genes Required for Hydrogenase Activity in Chlamydomonas reinhardtii

M. C. Posewitz, P. W. King, S. L. Smolinski, R. Davis Smith, A. R. Ginley, M. L. Ghirardi, M. Seibert

Research output: Contribution to journalArticlepeer-review

80 Scopus Citations

Abstract

The eukaryotic green alga, Chlamydamonas reinhardtii, produces H 2 under anaerobic conditions, in a reaction catalysed by an [FeFe]-hydrogenase. To identify genes that influence H2 production in C. reinhardtii, a library of 6000 colonies on agar plates was screened with sensitive chemochromic H2-sensor films for clones defective in H 2 production. Two mutants of particular interest were fully characterized. One mutant, hydEF-1, is unable to assemble an active [FeFe]-hydrogenase. This is the first reported C. reinhardtii mutant that is not capable of producing any H2. The second mutant, sta7-10, is not able to accumulate insoluble starch and has significantly lowered H 2-photoproduction rates in comparison with the wild-type. In hydEF-1, anaerobiosis induces transcription of the two reported C. reinhardtii hydrogenase genes, HydA1 and HydA2, indicating a normal transcriptional response to anaerobiosis. In contrast, the transcription of both hydrogenase genes in sta7-10 is significantly attenuated.

Original languageAmerican English
Pages (from-to)102-104
Number of pages3
JournalBiochemical Society Transactions
Volume33
Issue number1
DOIs
StatePublished - 2005

NREL Publication Number

  • NREL/JA-590-38657

Keywords

  • Chlamydomonas reinhardtii
  • H photoproduction
  • Hydrogenase
  • Insertional mutagenesis
  • Metalloprotein assembly
  • Starch

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