Identification of Microbial Inhibitory Functional Groups in Corn Stover Hydrolysate by Carbon-13 Nuclear Magnetic Resonance Spectroscopy

F. A. Agblevor; J. Fu; B. Hames; J. D. McMillan

doi:10.1385/ABAB:119:2:097

Identification of Microbial Inhibitory Functional Groups in Corn Stover Hydrolysate by Carbon-13 Nuclear Magnetic Resonance Spectroscopy

F. A. Agblevor, J. Fu, B. Hames, J. D. McMillan

BioEconomy and Sustainable Transportation

Research output: Contribution to journal › Article › peer-review

20 Scopus Citations

Abstract

Dilute-acid biomass hydrolysates contain biomass degradation products that are inhibitory to cell growth and fermentation. Overliming with Ca(OH) ₂ has been found to be one of the most effective methods for detoxifying the dilute-acid hydrolysate for ethanol production. However, the mechanism of overliming is not well understood. Carbon-13 nuclear magnetic resonance (¹³C-NMR) spectroscopy was used to elucidate the functional groups involved in the overliming reaction. The ¹³C-NMR spectra showed that the major functional groups removed during the overliming process were aliphatic and aromatic acids or esters, and other aromatic and aliphatic compounds. Ketone and aldehyde functionalities were not detected in the spectra. This is the first time that ¹³C-NMR has been used to elucidate the overliming reaction.

Original language	American English
Pages (from-to)	97-120
Number of pages	24
Journal	Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume	119
Issue number	2
DOIs	https://doi.org/10.1385/ABAB:119:2:097 https://doi.org/10.1385/abab:119:2:097
State	Published - 2004

NREL Publication Number

NREL/JA-510-34399

Keywords

Acetone
Carbon-13 nuclear magnetic resonance
Corn stover hydrolysate
Inhibition
Overliming

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Agblevor, F. A., Fu, J., Hames, B., & McMillan, J. D. (2004). Identification of Microbial Inhibitory Functional Groups in Corn Stover Hydrolysate by Carbon-13 Nuclear Magnetic Resonance Spectroscopy. Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology, 119(2), 97-120. https://doi.org/10.1385/ABAB:119:2:097, https://doi.org/10.1385/abab:119:2:097

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abstract = "Dilute-acid biomass hydrolysates contain biomass degradation products that are inhibitory to cell growth and fermentation. Overliming with Ca(OH) 2 has been found to be one of the most effective methods for detoxifying the dilute-acid hydrolysate for ethanol production. However, the mechanism of overliming is not well understood. Carbon-13 nuclear magnetic resonance (13C-NMR) spectroscopy was used to elucidate the functional groups involved in the overliming reaction. The 13C-NMR spectra showed that the major functional groups removed during the overliming process were aliphatic and aromatic acids or esters, and other aromatic and aliphatic compounds. Ketone and aldehyde functionalities were not detected in the spectra. This is the first time that 13C-NMR has been used to elucidate the overliming reaction.",

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Agblevor, FA, Fu, J, Hames, B & McMillan, JD 2004, 'Identification of Microbial Inhibitory Functional Groups in Corn Stover Hydrolysate by Carbon-13 Nuclear Magnetic Resonance Spectroscopy', Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology, vol. 119, no. 2, pp. 97-120. https://doi.org/10.1385/ABAB:119:2:097, https://doi.org/10.1385/abab:119:2:097

Identification of Microbial Inhibitory Functional Groups in Corn Stover Hydrolysate by Carbon-13 Nuclear Magnetic Resonance Spectroscopy. / Agblevor, F. A.; Fu, J.; Hames, B. et al.
In: Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology, Vol. 119, No. 2, 2004, p. 97-120.

Research output: Contribution to journal › Article › peer-review

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T1 - Identification of Microbial Inhibitory Functional Groups in Corn Stover Hydrolysate by Carbon-13 Nuclear Magnetic Resonance Spectroscopy

AU - Agblevor, F. A.

AU - Fu, J.

AU - Hames, B.

AU - McMillan, J. D.

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AB - Dilute-acid biomass hydrolysates contain biomass degradation products that are inhibitory to cell growth and fermentation. Overliming with Ca(OH) 2 has been found to be one of the most effective methods for detoxifying the dilute-acid hydrolysate for ethanol production. However, the mechanism of overliming is not well understood. Carbon-13 nuclear magnetic resonance (13C-NMR) spectroscopy was used to elucidate the functional groups involved in the overliming reaction. The 13C-NMR spectra showed that the major functional groups removed during the overliming process were aliphatic and aromatic acids or esters, and other aromatic and aliphatic compounds. Ketone and aldehyde functionalities were not detected in the spectra. This is the first time that 13C-NMR has been used to elucidate the overliming reaction.

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Identification of Microbial Inhibitory Functional Groups in Corn Stover Hydrolysate by Carbon-13 Nuclear Magnetic Resonance Spectroscopy

Abstract

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Keywords

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