Label-Free, Real-Time Monitoring of Biomass Processing with Stimulated Raman Scattering Microscopy

Brian G. Saar, Yining Zeng, Christian W. Freudiger, Yu San Liu, Michael E. Himmel, X. Sunney Xie, Shi You Ding

Research output: Contribution to journalArticlepeer-review

95 Scopus Citations

Abstract

(Figure Presented) Label-free "chemical movies" of lignin and cellulose in fresh plant tissue were recorded during a step in the breakdown of biomass to produce biofuels by using stimulated Raman scattering microscopy. The technique allows the degradation of lignin in the cell wall to be monitored with high sensitivity, sub-micrometer spatial resolution, and high temporal resolution.

Original languageAmerican English
Pages (from-to)5476-5479
Number of pages4
JournalAngewandte Chemie - International Edition
Volume49
Issue number32
DOIs
StatePublished - 2010

NREL Publication Number

  • NREL/JA-270-49266

Keywords

  • Biofuels
  • Cellulose
  • Delignification nonlinear optics
  • Raman spectroscopy

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