Life Cycle of a Cyanobacterial Carboxysome: Article No. eaba1269

Nicholas Hill, Jian Tay, Sabina Altus, David Bortz, Jeffrey Cameron

Research output: Contribution to journalArticlepeer-review

37 Scopus Citations

Abstract

Carboxysomes, prototypical bacterial microcompartments (BMCs) found in cyanobacteria, are large (~1 GDa) and essential protein complexes that enhance CO2 fixation. While carboxysome biogenesis has been elucidated, the activity dynamics, lifetime, and degradation of these structures have not been investigated, owing to the inability of tracking individual BMCs over time in vivo. We have developed a fluorescence-imaging platform to simultaneously measure carboxysome number, position, and activity over time in a growing cyanobacterial population, allowing individual carboxysomes to be clustered on the basis of activity and spatial dynamics. We have demonstrated both BMC degradation, characterized by abrupt activity loss followed by polar recruitment of the deactivated complex, and a subclass of ultraproductive carboxysomes. Together, our results reveal the BMC life cycle after biogenesis and describe the first method for measuring activity of single BMCs in vivo.
Original languageAmerican English
Number of pages8
JournalScience Advances
Volume6
Issue number19
DOIs
StatePublished - 2020

NREL Publication Number

  • NREL/JA-2800-77244

Keywords

  • activity dynamics
  • activity loss
  • carboxysomes
  • CO2 fixation
  • cyanobacterial population
  • essential proteins
  • micro-compartments
  • spatial dynamics

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