Low molecular weight thermostable β-D-glucosidase from Acidothermus cellulolyticus

W. S. Adney, M. P. Tucker, R. A. Nieves, S. R. Thomas, M. E. Himmel

Research output: Contribution to journalArticlepeer-review

4 Scopus Citations


Industrial use of cellulase enzymes, including β-D-glucosidases, can be greatly improved and expanded by the discovery and characterization of more robust, thermotolerant enzymes. A new thermostable β-D-glucosidase was isolated from Acidothermus cellulolyticus culture broth. This enzyme has a molecular weight of 52.5 kDa, an isoelectric pI of 4.1, and a pH activity optimum of 4.5. The optimum temperature for activity on p-nitrophenyl-β-D-glucoside is 63°C. This value is approximately 20°C higher than temperature optima displayed by most previously characterized β-D-glucosidases.

Original languageAmerican English
Pages (from-to)49-54
Number of pages6
JournalBiotechnology Letters
Issue number1
StatePublished - 1995

NREL Publication Number

  • NREL/JA-422-7312


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