MBTH: A Novel Approach to Rapid, Spectrophotometric Quantitation of Total Algal Carbohydrates

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20 Scopus Citations

Abstract

A high-throughput and robust application of the 3-methyl-2-benzothiazolinone hydrazone (MBTH) method was developed for carbohydrate determination in microalgae. The traditional phenol-sulfuric acid method to quantify carbohydrates is strongly affected by algal biochemical components and exhibits a highly variable response to microalgal monosaccharides. We present a novel use of the MBTH method to accurately quantify carbohydrates in hydrolyzate after acid hydrolysis of algal biomass, without a need for neutralization. The MBTH method demonstrated consistent and sensitive quantitation of algae-specific monosaccharides down to 5 μg mL−1 without interference from other algae acidic hydrolyzate components.

Original languageAmerican English
Pages (from-to)90-93
Number of pages4
JournalAnalytical Biochemistry
Volume518
DOIs
StatePublished - 2017

Bibliographical note

Publisher Copyright:
© 2016 Elsevier Inc.

NREL Publication Number

  • NREL/JA-5100-67563

Keywords

  • Algae
  • Carbohydrates
  • MBTH
  • Phenol-sulfuric acid
  • Spectrophotometric

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