MBTH: A Novel Approach to Rapid, Spectrophotometric Quantitation of Total Algal Carbohydrates

Stefanie Van Wychen; Stuart Black; Lieve Laurens

doi:10.1016/j.ab.2016.11.014

MBTH: A Novel Approach to Rapid, Spectrophotometric Quantitation of Total Algal Carbohydrates

Stefanie Van Wychen, Stuart Black, Lieve Laurens

Research output: Contribution to journal › Article › peer-review

23 Scopus Citations

Abstract

A high-throughput and robust application of the 3-methyl-2-benzothiazolinone hydrazone (MBTH) method was developed for carbohydrate determination in microalgae. The traditional phenol-sulfuric acid method to quantify carbohydrates is strongly affected by algal biochemical components and exhibits a highly variable response to microalgal monosaccharides. We present a novel use of the MBTH method to accurately quantify carbohydrates in hydrolyzate after acid hydrolysis of algal biomass, without a need for neutralization. The MBTH method demonstrated consistent and sensitive quantitation of algae-specific monosaccharides down to 5 μg mL⁻¹ without interference from other algae acidic hydrolyzate components.

Original language	American English
Pages (from-to)	90-93
Number of pages	4
Journal	Analytical Biochemistry
Volume	518
DOIs	https://doi.org/10.1016/j.ab.2016.11.014 https://doi.org/10.1016/j.ab.2016.11.014
State	Published - 2017

Bibliographical note

Publisher Copyright:
© 2016 Elsevier Inc.

NREL Publication Number

NREL/JA-5100-67563

Keywords

Algae
Carbohydrates
MBTH
Phenol-sulfuric acid
Spectrophotometric

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title = "MBTH: A Novel Approach to Rapid, Spectrophotometric Quantitation of Total Algal Carbohydrates",

abstract = "A high-throughput and robust application of the 3-methyl-2-benzothiazolinone hydrazone (MBTH) method was developed for carbohydrate determination in microalgae. The traditional phenol-sulfuric acid method to quantify carbohydrates is strongly affected by algal biochemical components and exhibits a highly variable response to microalgal monosaccharides. We present a novel use of the MBTH method to accurately quantify carbohydrates in hydrolyzate after acid hydrolysis of algal biomass, without a need for neutralization. The MBTH method demonstrated consistent and sensitive quantitation of algae-specific monosaccharides down to 5 μg mL−1 without interference from other algae acidic hydrolyzate components.",

keywords = "Algae, Carbohydrates, MBTH, Phenol-sulfuric acid, Spectrophotometric",

author = "{Van Wychen}, Stefanie and Stuart Black and Lieve Laurens",

note = "Publisher Copyright: {\textcopyright} 2016 Elsevier Inc.",

year = "2017",

doi = "10.1016/j.ab.2016.11.014",

language = "American English",

volume = "518",

pages = "90--93",

journal = "Analytical Biochemistry",

issn = "0003-2697",

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T1 - MBTH: A Novel Approach to Rapid, Spectrophotometric Quantitation of Total Algal Carbohydrates

AU - Van Wychen, Stefanie

AU - Black, Stuart

AU - Laurens, Lieve

PY - 2017

Y1 - 2017

N2 - A high-throughput and robust application of the 3-methyl-2-benzothiazolinone hydrazone (MBTH) method was developed for carbohydrate determination in microalgae. The traditional phenol-sulfuric acid method to quantify carbohydrates is strongly affected by algal biochemical components and exhibits a highly variable response to microalgal monosaccharides. We present a novel use of the MBTH method to accurately quantify carbohydrates in hydrolyzate after acid hydrolysis of algal biomass, without a need for neutralization. The MBTH method demonstrated consistent and sensitive quantitation of algae-specific monosaccharides down to 5 μg mL−1 without interference from other algae acidic hydrolyzate components.

AB - A high-throughput and robust application of the 3-methyl-2-benzothiazolinone hydrazone (MBTH) method was developed for carbohydrate determination in microalgae. The traditional phenol-sulfuric acid method to quantify carbohydrates is strongly affected by algal biochemical components and exhibits a highly variable response to microalgal monosaccharides. We present a novel use of the MBTH method to accurately quantify carbohydrates in hydrolyzate after acid hydrolysis of algal biomass, without a need for neutralization. The MBTH method demonstrated consistent and sensitive quantitation of algae-specific monosaccharides down to 5 μg mL−1 without interference from other algae acidic hydrolyzate components.

KW - Algae

KW - Carbohydrates

KW - MBTH

KW - Phenol-sulfuric acid

KW - Spectrophotometric

UR - http://www.scopus.com/inward/record.url?scp=84999233568&partnerID=8YFLogxK

U2 - 10.1016/j.ab.2016.11.014

DO - 10.1016/j.ab.2016.11.014

M3 - Article

C2 - 27890408

AN - SCOPUS:84999233568

SN - 0003-2697

VL - 518

SP - 90

EP - 93

JO - Analytical Biochemistry

JF - Analytical Biochemistry

ER -

MBTH: A Novel Approach to Rapid, Spectrophotometric Quantitation of Total Algal Carbohydrates

Abstract

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Keywords

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