Membrane Permeability of Fatty Acyl Compounds Studied via Molecular Simulation

Research output: Contribution to journalArticlepeer-review

19 Scopus Citations

Abstract

Interest in fatty acid-derived products as fuel and chemical precursors has grown substantially. Microbes can be genetically engineered to produce fatty acid-derived products that are able to cross host membranes and can be extracted into an applied organic overlay. This process is thought to be passive, with a rate dependent on the chemistry of the crossing compound. The relationship between the chemical composition and the energetics and kinetics of product accumulation within the overlay is not well understood. Through biased and unbiased molecular simulation, we compute the membrane permeability coefficients from production to extraction for different fatty acyl products, including fatty acids, fatty alcohols, fatty aldehydes, alkanes, and alkenes. These simulations identify specific interactions that accelerate the transit of aldehydes across the membrane bilayer relative to other oxidized products, specifically the lack of hydrogen bonds to the surrounding membrane environment. However, since extraction from the outer membrane leaflet into the organic phase is found to be rate limiting for the entire process, we find that fatty alcohols and fatty aldehydes would both manifest similar fluxes into a dodecane overlay under equivalent conditions, outpacing the accumulation of acids or alkanes into the organic phase. Since aldehydes are known to be highly reactive as well as toxic in high quantities, the findings suggest that indeed fatty alcohols are the optimal long-tail fatty acyl product for extraction.

Original languageAmerican English
Pages (from-to)11311-11324
Number of pages14
JournalJournal of Physical Chemistry B
Volume121
Issue number50
DOIs
StatePublished - 21 Dec 2017

Bibliographical note

Publisher Copyright:
© 2017 American Chemical Society.

NREL Publication Number

  • NREL/JA-2700-69128

Keywords

  • biochemistry
  • permeability
  • precursors

Fingerprint

Dive into the research topics of 'Membrane Permeability of Fatty Acyl Compounds Studied via Molecular Simulation'. Together they form a unique fingerprint.

Cite this