Metabolic Engineering of a Pentose Metabolism Pathway in Ethanologenic Zymomonas mobilis

Min Zhang, Christina Eddy, Kristine Deanda, Mark Finkelstein, Stephen Picataggio

Research output: Contribution to journalArticlepeer-review

595 Scopus Citations

Abstract

The ethanol-producing bacterium Zymomonas mobilis was metabolically engineered to broaden its range of fermentable substrates to include the pentose sugar xylose. Two operons encoding xylose assimilation and pentose phosphate pathway enzymes were constructed and transformed into Z. mobilis in order to generate a strain that grew on xylose and efficiently fermented it to ethanol. Thus, anaerobic fermentation of a pentose sugar to ethanol was achieved through a combination of the pentose phosphate and Entner-Doudoroff pathways. Furthermore, this strain efficiently fermented both glucose and xylose, which is essential for economical conversion of lignocellulosic biomass to ethanol.

Original languageAmerican English
Pages (from-to)240-243
Number of pages4
JournalScience
Volume267
Issue number5195
DOIs
StatePublished - 1995

NREL Publication Number

  • ACNR/JA-422-14865

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