Metabolic Profiling of Two White-Rot Fungi During 4-Hydroxybenzoate Conversion Reveals Biotechnologically Relevant Biosynthetic Pathways: Article No. 224

Lummy Maria Oliveira Monteiro; Carlos del Cerro; Teeratas Kijpornyongpan; Allison Yaguchi; Anna Bennett; Bryon Donohoe; Kelsey Ramirez; Alex Benson; Hugh Mitchell; Samuel Purvine; Lye Meng Markillie; Meagan Burnet; Kent Bloodsworth; Benjamin Bowen; Thomas Harwood; Katherine Louie; Trent Northen; Davinia Salvachua

doi:10.1038/s42003-025-07640-9

Metabolic Profiling of Two White-Rot Fungi During 4-Hydroxybenzoate Conversion Reveals Biotechnologically Relevant Biosynthetic Pathways: Article No. 224

Lummy Maria Oliveira Monteiro, Carlos del Cerro, Teeratas Kijpornyongpan, Allison Yaguchi, Anna Bennett, Bryon Donohoe, Kelsey Ramirez, Alex Benson, Hugh Mitchell, Samuel Purvine, Lye Meng Markillie, Meagan Burnet, Kent Bloodsworth, Benjamin Bowen, Thomas Harwood, Katherine Louie, Trent Northen, Davinia Salvachua

Research output: Contribution to journal › Article › peer-review

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Abstract

White-rot fungi are efficient organisms for the mineralization of lignin and polysaccharides into CO2 and H2O. Despite their biotechnological potential, WRF metabolism remains underexplored. Building on recent findings regarding the utilization of lignin-related aromatic compounds as carbon sources by WRF, we aimed to gain further insights into these catabolic processes. For this purpose, Trametes versicolor and Gelatoporia subvermispora were incubated in varying conditions - in static and agitation modes and different antioxidant levels - during the conversion of 4-hydroxybenzoic acid (a lignin-related compound) and cellobiose. Their metabolic responses were assessed via transcriptomics, proteomics, lipidomics, metabolomics, and microscopy analyses. These analyses reveal the significant impact of cultivation conditions on sugar and aromatic catabolic pathways, as well as lipid composition of the fungal mycelia. Additionally, this study identifies biosynthetic pathways for the production of extracellular fatty acids and phenylpropanoids - both products with relevance in biotechnological applications - and provides insights into carbon fate in nature.

Original language	American English
Number of pages	15
Journal	Communications Biology
Volume	8
DOIs	https://doi.org/10.1038/s42003-025-07640-9
State	Published - 2025

NREL Publication Number

NREL/JA-2800-91611

Keywords

antioxidants
aromatic compounds
Gelatoporia subvermispora
hydroxyquinol
lignin
lipids
systems biology
Trametes versicolor

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10.1038/s42003-025-07640-9

https://www.nrel.gov/docs/fy25osti/91611.pdf

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Oliveira Monteiro, L. M., del Cerro, C., Kijpornyongpan, T., Yaguchi, A., Bennett, A., Donohoe, B., Ramirez, K., Benson, A., Mitchell, H., Purvine, S., Markillie, L. M., Burnet, M., Bloodsworth, K., Bowen, B., Harwood, T., Louie, K., Northen, T., & Salvachua, D. (2025). Metabolic Profiling of Two White-Rot Fungi During 4-Hydroxybenzoate Conversion Reveals Biotechnologically Relevant Biosynthetic Pathways: Article No. 224. Communications Biology, 8. https://doi.org/10.1038/s42003-025-07640-9

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title = "Metabolic Profiling of Two White-Rot Fungi During 4-Hydroxybenzoate Conversion Reveals Biotechnologically Relevant Biosynthetic Pathways: Article No. 224",

abstract = "White-rot fungi are efficient organisms for the mineralization of lignin and polysaccharides into CO2 and H2O. Despite their biotechnological potential, WRF metabolism remains underexplored. Building on recent findings regarding the utilization of lignin-related aromatic compounds as carbon sources by WRF, we aimed to gain further insights into these catabolic processes. For this purpose, Trametes versicolor and Gelatoporia subvermispora were incubated in varying conditions - in static and agitation modes and different antioxidant levels - during the conversion of 4-hydroxybenzoic acid (a lignin-related compound) and cellobiose. Their metabolic responses were assessed via transcriptomics, proteomics, lipidomics, metabolomics, and microscopy analyses. These analyses reveal the significant impact of cultivation conditions on sugar and aromatic catabolic pathways, as well as lipid composition of the fungal mycelia. Additionally, this study identifies biosynthetic pathways for the production of extracellular fatty acids and phenylpropanoids - both products with relevance in biotechnological applications - and provides insights into carbon fate in nature.",

keywords = "antioxidants, aromatic compounds, Gelatoporia subvermispora, hydroxyquinol, lignin, lipids, systems biology, Trametes versicolor",

author = "{Oliveira Monteiro}, {Lummy Maria} and {del Cerro}, Carlos and Teeratas Kijpornyongpan and Allison Yaguchi and Anna Bennett and Bryon Donohoe and Kelsey Ramirez and Alex Benson and Hugh Mitchell and Samuel Purvine and Markillie, {Lye Meng} and Meagan Burnet and Kent Bloodsworth and Benjamin Bowen and Thomas Harwood and Katherine Louie and Trent Northen and Davinia Salvachua",

year = "2025",

doi = "10.1038/s42003-025-07640-9",

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journal = "Communications Biology",

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Oliveira Monteiro, LM, del Cerro, C, Kijpornyongpan, T, Yaguchi, A, Bennett, A, Donohoe, B , Ramirez, K , Benson, A, Mitchell, H, Purvine, S, Markillie, LM, Burnet, M, Bloodsworth, K, Bowen, B, Harwood, T, Louie, K, Northen, T & Salvachua, D 2025, 'Metabolic Profiling of Two White-Rot Fungi During 4-Hydroxybenzoate Conversion Reveals Biotechnologically Relevant Biosynthetic Pathways: Article No. 224', Communications Biology, vol. 8. https://doi.org/10.1038/s42003-025-07640-9

TY - JOUR

T1 - Metabolic Profiling of Two White-Rot Fungi During 4-Hydroxybenzoate Conversion Reveals Biotechnologically Relevant Biosynthetic Pathways

T2 - Article No. 224

AU - Oliveira Monteiro, Lummy Maria

AU - del Cerro, Carlos

AU - Kijpornyongpan, Teeratas

AU - Yaguchi, Allison

AU - Bennett, Anna

AU - Donohoe, Bryon

AU - Ramirez, Kelsey

AU - Benson, Alex

AU - Mitchell, Hugh

AU - Purvine, Samuel

AU - Markillie, Lye Meng

AU - Burnet, Meagan

AU - Bloodsworth, Kent

AU - Bowen, Benjamin

AU - Harwood, Thomas

AU - Louie, Katherine

AU - Northen, Trent

AU - Salvachua, Davinia

PY - 2025

Y1 - 2025

N2 - White-rot fungi are efficient organisms for the mineralization of lignin and polysaccharides into CO2 and H2O. Despite their biotechnological potential, WRF metabolism remains underexplored. Building on recent findings regarding the utilization of lignin-related aromatic compounds as carbon sources by WRF, we aimed to gain further insights into these catabolic processes. For this purpose, Trametes versicolor and Gelatoporia subvermispora were incubated in varying conditions - in static and agitation modes and different antioxidant levels - during the conversion of 4-hydroxybenzoic acid (a lignin-related compound) and cellobiose. Their metabolic responses were assessed via transcriptomics, proteomics, lipidomics, metabolomics, and microscopy analyses. These analyses reveal the significant impact of cultivation conditions on sugar and aromatic catabolic pathways, as well as lipid composition of the fungal mycelia. Additionally, this study identifies biosynthetic pathways for the production of extracellular fatty acids and phenylpropanoids - both products with relevance in biotechnological applications - and provides insights into carbon fate in nature.

AB - White-rot fungi are efficient organisms for the mineralization of lignin and polysaccharides into CO2 and H2O. Despite their biotechnological potential, WRF metabolism remains underexplored. Building on recent findings regarding the utilization of lignin-related aromatic compounds as carbon sources by WRF, we aimed to gain further insights into these catabolic processes. For this purpose, Trametes versicolor and Gelatoporia subvermispora were incubated in varying conditions - in static and agitation modes and different antioxidant levels - during the conversion of 4-hydroxybenzoic acid (a lignin-related compound) and cellobiose. Their metabolic responses were assessed via transcriptomics, proteomics, lipidomics, metabolomics, and microscopy analyses. These analyses reveal the significant impact of cultivation conditions on sugar and aromatic catabolic pathways, as well as lipid composition of the fungal mycelia. Additionally, this study identifies biosynthetic pathways for the production of extracellular fatty acids and phenylpropanoids - both products with relevance in biotechnological applications - and provides insights into carbon fate in nature.

KW - antioxidants

KW - aromatic compounds

KW - Gelatoporia subvermispora

KW - hydroxyquinol

KW - lignin

KW - lipids

KW - systems biology

KW - Trametes versicolor

U2 - 10.1038/s42003-025-07640-9

DO - 10.1038/s42003-025-07640-9

M3 - Article

SN - 2399-3642

VL - 8

JO - Communications Biology

JF - Communications Biology

ER -

Metabolic Profiling of Two White-Rot Fungi During 4-Hydroxybenzoate Conversion Reveals Biotechnologically Relevant Biosynthetic Pathways: Article No. 224

Abstract

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Keywords

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