Metaproteomics Reveals Enzymatic Strategies Deployed by Anaerobic Microbiomes to Maintain Lignocellulose Deconstruction at High Solids

Payal Chirania, Evert Holwerda, Richard Giannone, Xiaoyu Liang, Suresh Poudel, Joseph Ellis, Yannick Bomble, Robert Hettich, Lee Lynd

Research output: Contribution to journalArticlepeer-review

16 Scopus Citations

Abstract

Economically viable production of cellulosic biofuels requires operation at high solids loadings—on the order of 15 wt%. To this end we characterize Nature’s ability to deconstruct and utilize mid-season switchgrass at increasing solid loadings using an anaerobic methanogenic microbiome. This community exhibits undiminished fractional carbohydrate solubilization at loadings ranging from 30 g/L to 150 g/L. Metaproteomic interrogation reveals marked increases in the abundance of specific carbohydrate-active enzyme classes. Significant enrichment of auxiliary activity family 6 enzymes at higher solids suggests a role for Fenton chemistry. Stress-response proteins accompanying these reactions are similarly upregulated at higher solids, as are β-glucosidases, xylosidases, carbohydrate-debranching, and pectin-acting enzymes—all of which indicate that removal of deconstruction inhibitors is important for observed undiminished solubilization. Our work provides insights into the mechanisms by which natural microbiomes effectively deconstruct and utilize lignocellulose at high solids loadings, informing the future development of defined cultures for efficient bioconversion.

Original languageAmerican English
Article numberArticle No. 3870
Number of pages13
JournalNature Communications
Volume13
Issue number1
DOIs
StatePublished - Dec 2022

Bibliographical note

Publisher Copyright:
© 2022, The Author(s).

NREL Publication Number

  • NREL/JA-2700-83070

Keywords

  • CAZymes
  • Fenton chemistry
  • high solids
  • lignocellulose
  • microbiomes

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