Abstract
Glucose yield from the enzymatic hydrolysis of cellulose was investigated as a function of cellulase enzyme loading (7-36 filter paper units [FPU]/g cellulose) and solids concentration (7-18% total solids) for up to 72 h on dilute sulfuric-acid pretreated Douglas Fir. The saccharification was performed on whole hydrolysate with no separation or washing of the solids. Enzyme loading had a significant effect on glucose yield; solids concentration had a much smaller effect ever at higher glucose concentrations. The data were used to generate an empirical model for glucose yield, and to fit parameters of a cellulose hydrolysis kinetic model. Both models could be used for economic evaluation of a separate hydrolysis and fermentation process.
Original language | American English |
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Pages (from-to) | 67-81 |
Number of pages | 15 |
Journal | Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology |
Volume | 77-79 |
DOIs | |
State | Published - 1999 |
NREL Publication Number
- NREL/JA-580-24673
Keywords
- Cellulose
- Cellulose hydrolysis
- Enzyme kinetics
- Ethanol
- Softwood