Modeling the Enzymatic Hydrolysis of Dilute-Acid Pretreated Douglas Fir

Daniel J. Schell, Mark F. Ruth, Melvin P. Tucker

Research output: Contribution to journalArticlepeer-review

39 Scopus Citations

Abstract

Glucose yield from the enzymatic hydrolysis of cellulose was investigated as a function of cellulase enzyme loading (7-36 filter paper units [FPU]/g cellulose) and solids concentration (7-18% total solids) for up to 72 h on dilute sulfuric-acid pretreated Douglas Fir. The saccharification was performed on whole hydrolysate with no separation or washing of the solids. Enzyme loading had a significant effect on glucose yield; solids concentration had a much smaller effect ever at higher glucose concentrations. The data were used to generate an empirical model for glucose yield, and to fit parameters of a cellulose hydrolysis kinetic model. Both models could be used for economic evaluation of a separate hydrolysis and fermentation process.

Original languageAmerican English
Pages (from-to)67-81
Number of pages15
JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume77-79
DOIs
StatePublished - 1999

NREL Publication Number

  • NREL/JA-580-24673

Keywords

  • Cellulose
  • Cellulose hydrolysis
  • Enzyme kinetics
  • Ethanol
  • Softwood

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