Modeling the Enzymatic Hydrolysis of Dilute-Acid Pretreated Douglas Fir

Daniel J. Schell; Mark F. Ruth; Melvin P. Tucker

doi:10.1385/ABAB:77:1-3:67

Modeling the Enzymatic Hydrolysis of Dilute-Acid Pretreated Douglas Fir

Daniel J. Schell
, Mark F. Ruth
, Melvin P. Tucker

National Renewable Energy Laboratory

Research output: Contribution to journal › Article › peer-review

42 Scopus Citations

Abstract

Glucose yield from the enzymatic hydrolysis of cellulose was investigated as a function of cellulase enzyme loading (7-36 filter paper units [FPU]/g cellulose) and solids concentration (7-18% total solids) for up to 72 h on dilute sulfuric-acid pretreated Douglas Fir. The saccharification was performed on whole hydrolysate with no separation or washing of the solids. Enzyme loading had a significant effect on glucose yield; solids concentration had a much smaller effect ever at higher glucose concentrations. The data were used to generate an empirical model for glucose yield, and to fit parameters of a cellulose hydrolysis kinetic model. Both models could be used for economic evaluation of a separate hydrolysis and fermentation process.

Original language	American English
Pages (from-to)	67-81
Number of pages	15
Journal	Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume	77-79
DOIs	https://doi.org/10.1385/ABAB:77:1-3:67 https://doi.org/10.1385/abab:77:1-3:67
State	Published - 1999

NLR Publication Number

NREL/JA-580-24673

Keywords

Cellulose
Cellulose hydrolysis
Enzyme kinetics
Ethanol
Softwood

Access to Document

Cite this

@article{c960d2f863ff4e5ab9d8d128820a6fec,

title = "Modeling the Enzymatic Hydrolysis of Dilute-Acid Pretreated Douglas Fir",

abstract = "Glucose yield from the enzymatic hydrolysis of cellulose was investigated as a function of cellulase enzyme loading (7-36 filter paper units [FPU]/g cellulose) and solids concentration (7-18\% total solids) for up to 72 h on dilute sulfuric-acid pretreated Douglas Fir. The saccharification was performed on whole hydrolysate with no separation or washing of the solids. Enzyme loading had a significant effect on glucose yield; solids concentration had a much smaller effect ever at higher glucose concentrations. The data were used to generate an empirical model for glucose yield, and to fit parameters of a cellulose hydrolysis kinetic model. Both models could be used for economic evaluation of a separate hydrolysis and fermentation process.",

keywords = "Cellulose, Cellulose hydrolysis, Enzyme kinetics, Ethanol, Softwood",

author = "Schell, \{Daniel J.\} and Ruth, \{Mark F.\} and Tucker, \{Melvin P.\}",

year = "1999",

doi = "10.1385/ABAB:77:1-3:67",

language = "American English",

volume = "77-79",

pages = "67--81",

journal = "Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology",

issn = "0273-2289",

publisher = "Springer",

}

TY - JOUR

T1 - Modeling the Enzymatic Hydrolysis of Dilute-Acid Pretreated Douglas Fir

AU - Schell, Daniel J.

AU - Ruth, Mark F.

AU - Tucker, Melvin P.

PY - 1999

Y1 - 1999

N2 - Glucose yield from the enzymatic hydrolysis of cellulose was investigated as a function of cellulase enzyme loading (7-36 filter paper units [FPU]/g cellulose) and solids concentration (7-18% total solids) for up to 72 h on dilute sulfuric-acid pretreated Douglas Fir. The saccharification was performed on whole hydrolysate with no separation or washing of the solids. Enzyme loading had a significant effect on glucose yield; solids concentration had a much smaller effect ever at higher glucose concentrations. The data were used to generate an empirical model for glucose yield, and to fit parameters of a cellulose hydrolysis kinetic model. Both models could be used for economic evaluation of a separate hydrolysis and fermentation process.

AB - Glucose yield from the enzymatic hydrolysis of cellulose was investigated as a function of cellulase enzyme loading (7-36 filter paper units [FPU]/g cellulose) and solids concentration (7-18% total solids) for up to 72 h on dilute sulfuric-acid pretreated Douglas Fir. The saccharification was performed on whole hydrolysate with no separation or washing of the solids. Enzyme loading had a significant effect on glucose yield; solids concentration had a much smaller effect ever at higher glucose concentrations. The data were used to generate an empirical model for glucose yield, and to fit parameters of a cellulose hydrolysis kinetic model. Both models could be used for economic evaluation of a separate hydrolysis and fermentation process.

KW - Cellulose

KW - Cellulose hydrolysis

KW - Enzyme kinetics

KW - Ethanol

KW - Softwood

UR - https://www.scopus.com/pages/publications/0033063186

U2 - 10.1385/ABAB:77:1-3:67

DO - 10.1385/ABAB:77:1-3:67

M3 - Article

AN - SCOPUS:0033063186

SN - 0273-2289

VL - 77-79

SP - 67

EP - 81

JO - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

JF - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

ER -

Modeling the Enzymatic Hydrolysis of Dilute-Acid Pretreated Douglas Fir

Abstract

NLR Publication Number

Keywords

Access to Document

Other files and links

Fingerprint

Cite this