A new thermostable endoglucanase, Acidothermus cellulolyticus E1 - Synergism with Trichoderma reesei CBH I and comparison to Thermomonospora fusca E5

John O. Baker, William S. Adney, Rafael A. Nleves, Steven R. Thomas, David B. Wilson, Michael E. Himmel

Research output: Contribution to journalArticlepeer-review

51 Scopus Citations

Abstract

A new thermostable endoglucanase, Acidothermus cellulolyticus E1, and another bacterial endoglucanase, E5 from Thermomonospora fusca, each exhibit striking synergism with a fungal cellobiohydrolase (Trichoderma reesei CBH I) in the saccharification of microcrystalline cellulose. In neither case did the ratio of endoglucanase to exoglucanase that demonstrated maximum synergism coincide exactly with the ratio that actually released the maximum quantity of soluble sugar for a given total cellulase loading. The difference between the two ratios, after significant hydrolysis of the substrate, was considerably larger in the case of A. cellulolyticus E1. For both endoglucanase pairings with CBH I, the offset between the ratio for maximum synergism and the ratio for maximal soluble sugar production was found to be a function of digestion time.

Original languageAmerican English
Pages (from-to)245-256
Number of pages12
JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume45/46
Issue number1
DOIs
StatePublished - 1994

NREL Publication Number

  • NREL/JA-422-5470

Keywords

  • Acidothermus cellulolyticus E1
  • endoglucanase
  • synergism
  • Thermomonospora fusca E
  • Trichoderma reesei CBH I

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