TY - JOUR
T1 - Noble, Diatomic and Aliphatic Hydrocarbon Gas Analyses by Aqueous HPLC
AU - Baker, John O.
AU - Tucker, Melvin P.
AU - Himmel, Michael E.
PY - 1985
Y1 - 1985
N2 - Dissolved non-polar gases (five noble, three diatomic, and four unbranched aliphatic hydrocarbon gases) were resolved by aqueous high-performance liquid chromatography using an ion-moderated partition chromatography column (Bio-Rad HPX-87P) originally designed for monosaccharide analysis. The dissolved-gas peaks, which were detected and quantitated by high-sensitivity refractive-index detection, are eluted at 25°C in the order Ne, He, N2, H2, O2, Ar, CH4, Kr, Xe, C2H6, C3H8, n-C4H10. Based on the general elution order, and on the temperature dependences (4-72°C) of elution times for the individual gases, a mixed chromatographic mechanism is proposed, in which separation of the gases results from a mixture of size-exclusion, ion-ion-induced dipole, and classical hydrophobic effects, plus a second type of "hydrophobic" effect involving binding of the clathrate hydrate cages surrounding non-polar solutes to similar cages formed around hydrocarbon portions of the column packing material.
AB - Dissolved non-polar gases (five noble, three diatomic, and four unbranched aliphatic hydrocarbon gases) were resolved by aqueous high-performance liquid chromatography using an ion-moderated partition chromatography column (Bio-Rad HPX-87P) originally designed for monosaccharide analysis. The dissolved-gas peaks, which were detected and quantitated by high-sensitivity refractive-index detection, are eluted at 25°C in the order Ne, He, N2, H2, O2, Ar, CH4, Kr, Xe, C2H6, C3H8, n-C4H10. Based on the general elution order, and on the temperature dependences (4-72°C) of elution times for the individual gases, a mixed chromatographic mechanism is proposed, in which separation of the gases results from a mixture of size-exclusion, ion-ion-induced dipole, and classical hydrophobic effects, plus a second type of "hydrophobic" effect involving binding of the clathrate hydrate cages surrounding non-polar solutes to similar cages formed around hydrocarbon portions of the column packing material.
UR - http://www.scopus.com/inward/record.url?scp=46549103228&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(00)90497-7
DO - 10.1016/S0021-9673(00)90497-7
M3 - Article
AN - SCOPUS:46549103228
SN - 0021-9673
VL - 346
SP - 93
EP - 109
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - C
ER -