Oxygen-Resistant Hydrogenases and Methods for Designing and Making Same

Paul King; Michael Seibert; Maria Ghirardi

Oxygen-Resistant Hydrogenases and Methods for Designing and Making Same

Paul King (Inventor), Michael Seibert (Inventor), Maria Ghirardi (Inventor)

Research output: Patent

Abstract

The invention provides oxygen-resistant iron-hydrogenases ([Fe]-hydrogenases) for use in the production of H.sub.2. Methods used in the design and engineering of the oxygen-resistant [Fe]-hydrogenases are disclosed, as are the methods of transforming and culturing appropriate host cells with the oxygen-resistant [Fe]-hydrogenases. Finally, the invention provides methods for utilizing thetransformed, oxygen insensitive, host cells in the bulk production of H.sub.2 in a light catalyzed reaction having water as the reactant.

Original language	American English
Patent number	8,663,958
Filing date	4/03/14
State	Published - 2014

Bibliographical note

Assignee: Alliance for Sustainable Energy, LLC (Golden, CO)

NREL Publication Number

NREL/PT-2700-62966

Access to Document

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Cite this

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title = "Oxygen-Resistant Hydrogenases and Methods for Designing and Making Same",

abstract = "The invention provides oxygen-resistant iron-hydrogenases ([Fe]-hydrogenases) for use in the production of H.sub.2. Methods used in the design and engineering of the oxygen-resistant [Fe]-hydrogenases are disclosed, as are the methods of transforming and culturing appropriate host cells with the oxygen-resistant [Fe]-hydrogenases. Finally, the invention provides methods for utilizing thetransformed, oxygen insensitive, host cells in the bulk production of H.sub.2 in a light catalyzed reaction having water as the reactant.",

author = "Paul King and Michael Seibert and Maria Ghirardi",

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year = "2014",

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TY - PAT

T1 - Oxygen-Resistant Hydrogenases and Methods for Designing and Making Same

AU - King, Paul

AU - Seibert, Michael

AU - Ghirardi, Maria

N1 - Assignee: Alliance for Sustainable Energy, LLC (Golden, CO)

PY - 2014

Y1 - 2014

N2 - The invention provides oxygen-resistant iron-hydrogenases ([Fe]-hydrogenases) for use in the production of H.sub.2. Methods used in the design and engineering of the oxygen-resistant [Fe]-hydrogenases are disclosed, as are the methods of transforming and culturing appropriate host cells with the oxygen-resistant [Fe]-hydrogenases. Finally, the invention provides methods for utilizing thetransformed, oxygen insensitive, host cells in the bulk production of H.sub.2 in a light catalyzed reaction having water as the reactant.

AB - The invention provides oxygen-resistant iron-hydrogenases ([Fe]-hydrogenases) for use in the production of H.sub.2. Methods used in the design and engineering of the oxygen-resistant [Fe]-hydrogenases are disclosed, as are the methods of transforming and culturing appropriate host cells with the oxygen-resistant [Fe]-hydrogenases. Finally, the invention provides methods for utilizing thetransformed, oxygen insensitive, host cells in the bulk production of H.sub.2 in a light catalyzed reaction having water as the reactant.

M3 - Patent

M1 - 8,663,958

Y2 - 2014/03/04

ER -

Oxygen-Resistant Hydrogenases and Methods for Designing and Making Same

Abstract

Bibliographical note

NREL Publication Number

Access to Document

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Cite this