Oxygen-Resistant Hydrogenases and Methods for Designing and Making Same

Paul King (Inventor), Michael Seibert (Inventor), Maria Ghirardi (Inventor)

Research output: Patent


The invention provides oxygen-resistant iron-hydrogenases ([Fe]-hydrogenases) for use in the production of H.sub.2. Methods used in the design and engineering of the oxygen-resistant [Fe]-hydrogenases are disclosed, as are the methods of transforming and culturing appropriate host cells with the oxygen-resistant [Fe]-hydrogenases. Finally, the invention provides methods for utilizing thetransformed, oxygen insensitive, host cells in the bulk production of H.sub.2 in a light catalyzed reaction having water as the reactant.
Original languageAmerican English
Patent number8,663,958
Filing date4/03/14
StatePublished - 2014

Bibliographical note

Assignee: Alliance for Sustainable Energy, LLC (Golden, CO)

NREL Publication Number

  • NREL/PT-2700-62966


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