Photocatalytic Regeneration of Nicotinamide Cofactors by Quantum Dot-Enzyme Biohybrid Complexes

Katherine A. Brown; Molly B. Wilker; Marko Boehm; Hayden Hamby; Gordana Dukovic; Paul W. King

doi:10.1021/acscatal.5b02850

Photocatalytic Regeneration of Nicotinamide Cofactors by Quantum Dot-Enzyme Biohybrid Complexes

Katherine A. Brown, Molly B. Wilker, Marko Boehm, Hayden Hamby, Gordana Dukovic, Paul W. King

Biosciences Center

Research output: Contribution to journal › Article › peer-review

89 Scopus Citations

Abstract

We report the characterization of biohybrid complexes of CdSe quantum dots and ferredoxin NADP⁺-reductase for photocatalytic regeneration of NADPH. Illumination with visible light led to reduction of NADP⁺ to NADPH, with an apparent k_cat of 1400 h^-1. Regeneration of NADPH was coupled to reduction of aldehydes to alcohols catalyzed by a NADPH-dependent alcohol dehydrogenase, with each NADPH molecule recycled an average of 7.5 times. The quantum yield both of NADPH and alcohol production were 5-6% for both products. Light-driven NADPH regeneration was also demonstrated in a multienzyme system, showing the capacity of QD-FNR complexes to drive continuous NADPH-dependent transformations.

Original language	American English
Pages (from-to)	2201-2204
Number of pages	4
Journal	ACS Catalysis
Volume	6
Issue number	4
DOIs	https://doi.org/10.1021/acscatal.5b02850 https://doi.org/10.1021/acscatal.5b02850
State	Published - 1 Apr 2016

Bibliographical note

Publisher Copyright:
© 2016 American Chemical Society.

NREL Publication Number

NREL/JA-2700-65500

Keywords

biocatalysis
biohybrid
NADPH regeneration
photocatalysis
quantum dots

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abstract = "We report the characterization of biohybrid complexes of CdSe quantum dots and ferredoxin NADP+-reductase for photocatalytic regeneration of NADPH. Illumination with visible light led to reduction of NADP+ to NADPH, with an apparent kcat of 1400 h-1. Regeneration of NADPH was coupled to reduction of aldehydes to alcohols catalyzed by a NADPH-dependent alcohol dehydrogenase, with each NADPH molecule recycled an average of 7.5 times. The quantum yield both of NADPH and alcohol production were 5-6% for both products. Light-driven NADPH regeneration was also demonstrated in a multienzyme system, showing the capacity of QD-FNR complexes to drive continuous NADPH-dependent transformations.",

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T1 - Photocatalytic Regeneration of Nicotinamide Cofactors by Quantum Dot-Enzyme Biohybrid Complexes

AU - Brown, Katherine A.

AU - Wilker, Molly B.

AU - Boehm, Marko

AU - Hamby, Hayden

AU - Dukovic, Gordana

AU - King, Paul W.

PY - 2016/4/1

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N2 - We report the characterization of biohybrid complexes of CdSe quantum dots and ferredoxin NADP+-reductase for photocatalytic regeneration of NADPH. Illumination with visible light led to reduction of NADP+ to NADPH, with an apparent kcat of 1400 h-1. Regeneration of NADPH was coupled to reduction of aldehydes to alcohols catalyzed by a NADPH-dependent alcohol dehydrogenase, with each NADPH molecule recycled an average of 7.5 times. The quantum yield both of NADPH and alcohol production were 5-6% for both products. Light-driven NADPH regeneration was also demonstrated in a multienzyme system, showing the capacity of QD-FNR complexes to drive continuous NADPH-dependent transformations.

AB - We report the characterization of biohybrid complexes of CdSe quantum dots and ferredoxin NADP+-reductase for photocatalytic regeneration of NADPH. Illumination with visible light led to reduction of NADP+ to NADPH, with an apparent kcat of 1400 h-1. Regeneration of NADPH was coupled to reduction of aldehydes to alcohols catalyzed by a NADPH-dependent alcohol dehydrogenase, with each NADPH molecule recycled an average of 7.5 times. The quantum yield both of NADPH and alcohol production were 5-6% for both products. Light-driven NADPH regeneration was also demonstrated in a multienzyme system, showing the capacity of QD-FNR complexes to drive continuous NADPH-dependent transformations.

KW - biocatalysis

KW - biohybrid

KW - NADPH regeneration

KW - photocatalysis

KW - quantum dots

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Photocatalytic Regeneration of Nicotinamide Cofactors by Quantum Dot-Enzyme Biohybrid Complexes

Abstract

Bibliographical note

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Keywords

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