Photocatalytic Regeneration of Nicotinamide Cofactors by Quantum Dot-Enzyme Biohybrid Complexes

Katherine A. Brown, Molly B. Wilker, Marko Boehm, Hayden Hamby, Gordana Dukovic, Paul W. King

Research output: Contribution to journalArticlepeer-review

87 Scopus Citations


We report the characterization of biohybrid complexes of CdSe quantum dots and ferredoxin NADP+-reductase for photocatalytic regeneration of NADPH. Illumination with visible light led to reduction of NADP+ to NADPH, with an apparent kcat of 1400 h-1. Regeneration of NADPH was coupled to reduction of aldehydes to alcohols catalyzed by a NADPH-dependent alcohol dehydrogenase, with each NADPH molecule recycled an average of 7.5 times. The quantum yield both of NADPH and alcohol production were 5-6% for both products. Light-driven NADPH regeneration was also demonstrated in a multienzyme system, showing the capacity of QD-FNR complexes to drive continuous NADPH-dependent transformations.

Original languageAmerican English
Pages (from-to)2201-2204
Number of pages4
JournalACS Catalysis
Issue number4
StatePublished - 1 Apr 2016

Bibliographical note

Publisher Copyright:
© 2016 American Chemical Society.

NREL Publication Number

  • NREL/JA-2700-65500


  • biocatalysis
  • biohybrid
  • NADPH regeneration
  • photocatalysis
  • quantum dots


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