A Photosynthetic Photoelectrochemical Cell Using Phenazine Methosulfate and Phenazine Ethosulfate as Electron Acceptors

Douglas G. Sanderson; Elizabeth L. Gross; Michael Seibert

doi:10.1007/BF02798494

A Photosynthetic Photoelectrochemical Cell Using Phenazine Methosulfate and Phenazine Ethosulfate as Electron Acceptors

Douglas G. Sanderson, Elizabeth L. Gross, Michael Seibert

BioEconomy and Sustainable Transportation

Ohio State University

Research output: Contribution to journal › Article › peer-review

16 Scopus Citations

Abstract

Several recent studies have demonstrated that photosystem I (PSI), one of the two light-active complexes of photosynthesis, can be used as a light transducer in a biological photoelectrochemical cell. This paper examines the results of using phenazine methosulfate (PMS) and phenazine ethosulfate (PES) as an electron acceptor in such a cell. The PMS and PES have relatively high formal potentials compared to flavin mononucleotide (FMN) and other acceptors used in the past, yet the PMS and PES resulted in power outputs and conversion efficiencies second only to the use of FMN as an acceptor. The mechanism of action has been interpreted in terms of electroactive products of parent compounds formed during the normal function of the cell under illuminated conditions. For example, photolysis and cyclic voltammetry data demonstrate that pyocyanin (Py) [formal potential = -0.37 vs saturated calomel electrode (SCE) at pH 8.5], the photoproduct of PMS, is the electroactive species in cells containing PMS (formal potential = -0.19 vs SCE at pH 8.5). Similar phenomena were observed for PES and FMN. The power output of the cell results from about equal contributions from a cyclic photosynthetic component and a component caused by the direct photoreduction of the acceptor and reoxidation by sacrificial donors in the buffer. Future research directions are discussed in terms of designing cells that function purely in the photosynthetic or photochemical modes.

Original language	American English
Pages (from-to)	1-20
Number of pages	20
Journal	Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume	14
Issue number	1
DOIs	https://doi.org/10.1007/BF02798494 https://doi.org/10.1007/BF02798494
State	Published - 1987

Bibliographical note

Work performed by Departments of Chemistry and Biochemistry, Ohio State University, Columbus, Ohio and Solar Energy Research Institute, Golden, Colorado

NREL Publication Number

ACNR/JA-233-6859

Keywords

acceptors
electrochemistry
flavin mononucleotide
phenazine ethosulfate
phenazine methosulfate
photochemistry
Photoelectrochemical cell
photosystem I, photosynthesis
pyocyanin
solar cell

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title = "A Photosynthetic Photoelectrochemical Cell Using Phenazine Methosulfate and Phenazine Ethosulfate as Electron Acceptors",

abstract = "Several recent studies have demonstrated that photosystem I (PSI), one of the two light-active complexes of photosynthesis, can be used as a light transducer in a biological photoelectrochemical cell. This paper examines the results of using phenazine methosulfate (PMS) and phenazine ethosulfate (PES) as an electron acceptor in such a cell. The PMS and PES have relatively high formal potentials compared to flavin mononucleotide (FMN) and other acceptors used in the past, yet the PMS and PES resulted in power outputs and conversion efficiencies second only to the use of FMN as an acceptor. The mechanism of action has been interpreted in terms of electroactive products of parent compounds formed during the normal function of the cell under illuminated conditions. For example, photolysis and cyclic voltammetry data demonstrate that pyocyanin (Py) [formal potential = -0.37 vs saturated calomel electrode (SCE) at pH 8.5], the photoproduct of PMS, is the electroactive species in cells containing PMS (formal potential = -0.19 vs SCE at pH 8.5). Similar phenomena were observed for PES and FMN. The power output of the cell results from about equal contributions from a cyclic photosynthetic component and a component caused by the direct photoreduction of the acceptor and reoxidation by sacrificial donors in the buffer. Future research directions are discussed in terms of designing cells that function purely in the photosynthetic or photochemical modes.",

keywords = "acceptors, electrochemistry, flavin mononucleotide, phenazine ethosulfate, phenazine methosulfate, photochemistry, Photoelectrochemical cell, photosystem I, photosynthesis, pyocyanin, solar cell",

author = "Sanderson, {Douglas G.} and Gross, {Elizabeth L.} and Michael Seibert",

note = "Work performed by Departments of Chemistry and Biochemistry, Ohio State University, Columbus, Ohio and Solar Energy Research Institute, Golden, Colorado",

year = "1987",

doi = "10.1007/BF02798494",

language = "American English",

volume = "14",

pages = "1--20",

journal = "Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology",

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A Photosynthetic Photoelectrochemical Cell Using Phenazine Methosulfate and Phenazine Ethosulfate as Electron Acceptors. / Sanderson, Douglas G.; Gross, Elizabeth L.; Seibert, Michael.
In: Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology, Vol. 14, No. 1, 1987, p. 1-20.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - A Photosynthetic Photoelectrochemical Cell Using Phenazine Methosulfate and Phenazine Ethosulfate as Electron Acceptors

AU - Sanderson, Douglas G.

AU - Gross, Elizabeth L.

AU - Seibert, Michael

N1 - Work performed by Departments of Chemistry and Biochemistry, Ohio State University, Columbus, Ohio and Solar Energy Research Institute, Golden, Colorado

PY - 1987

Y1 - 1987

N2 - Several recent studies have demonstrated that photosystem I (PSI), one of the two light-active complexes of photosynthesis, can be used as a light transducer in a biological photoelectrochemical cell. This paper examines the results of using phenazine methosulfate (PMS) and phenazine ethosulfate (PES) as an electron acceptor in such a cell. The PMS and PES have relatively high formal potentials compared to flavin mononucleotide (FMN) and other acceptors used in the past, yet the PMS and PES resulted in power outputs and conversion efficiencies second only to the use of FMN as an acceptor. The mechanism of action has been interpreted in terms of electroactive products of parent compounds formed during the normal function of the cell under illuminated conditions. For example, photolysis and cyclic voltammetry data demonstrate that pyocyanin (Py) [formal potential = -0.37 vs saturated calomel electrode (SCE) at pH 8.5], the photoproduct of PMS, is the electroactive species in cells containing PMS (formal potential = -0.19 vs SCE at pH 8.5). Similar phenomena were observed for PES and FMN. The power output of the cell results from about equal contributions from a cyclic photosynthetic component and a component caused by the direct photoreduction of the acceptor and reoxidation by sacrificial donors in the buffer. Future research directions are discussed in terms of designing cells that function purely in the photosynthetic or photochemical modes.

AB - Several recent studies have demonstrated that photosystem I (PSI), one of the two light-active complexes of photosynthesis, can be used as a light transducer in a biological photoelectrochemical cell. This paper examines the results of using phenazine methosulfate (PMS) and phenazine ethosulfate (PES) as an electron acceptor in such a cell. The PMS and PES have relatively high formal potentials compared to flavin mononucleotide (FMN) and other acceptors used in the past, yet the PMS and PES resulted in power outputs and conversion efficiencies second only to the use of FMN as an acceptor. The mechanism of action has been interpreted in terms of electroactive products of parent compounds formed during the normal function of the cell under illuminated conditions. For example, photolysis and cyclic voltammetry data demonstrate that pyocyanin (Py) [formal potential = -0.37 vs saturated calomel electrode (SCE) at pH 8.5], the photoproduct of PMS, is the electroactive species in cells containing PMS (formal potential = -0.19 vs SCE at pH 8.5). Similar phenomena were observed for PES and FMN. The power output of the cell results from about equal contributions from a cyclic photosynthetic component and a component caused by the direct photoreduction of the acceptor and reoxidation by sacrificial donors in the buffer. Future research directions are discussed in terms of designing cells that function purely in the photosynthetic or photochemical modes.

KW - acceptors

KW - electrochemistry

KW - flavin mononucleotide

KW - phenazine ethosulfate

KW - phenazine methosulfate

KW - photochemistry

KW - Photoelectrochemical cell

KW - photosystem I, photosynthesis

KW - pyocyanin

KW - solar cell

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U2 - 10.1007/BF02798494

DO - 10.1007/BF02798494

M3 - Article

AN - SCOPUS:51649151956

SN - 0273-2289

VL - 14

SP - 1

EP - 20

JO - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

JF - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

IS - 1

ER -

A Photosynthetic Photoelectrochemical Cell Using Phenazine Methosulfate and Phenazine Ethosulfate as Electron Acceptors

Abstract

Bibliographical note

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Keywords

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