Abstract
Xylose isomerase was purified from a transformed E. coli strain (LE392-pRK248/pTXI-1) (Lastick et al., 1986) that overproduces the enzyme by induction of the strong lambda PL promotor. Kinetic data, N-terminal sequence analysis, SDS polyacrylamide gel electrophoresis, size exclusion chromatography and immunodiffusion were used to compare the overproduced enzyme with xylose isomerase purified from xylose induced, non-transformed E. coli LE392 cells; no differences between these purified enzyme preparations were found.
Original language | American English |
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Pages (from-to) | 79-84 |
Number of pages | 6 |
Journal | Biotechnology Letters |
Volume | 10 |
Issue number | 2 |
DOIs | |
State | Published - 1988 |
NREL Publication Number
- ACNR/JA-232-10212