Properties of Genetically Overproduced E. coli Xylose Isomerase

M. Y. Tucker, M. P. Tucker, M. E. Himmel, K. Grohmann, S. M. Lastick

Research output: Contribution to journalArticlepeer-review

6 Scopus Citations


Xylose isomerase was purified from a transformed E. coli strain (LE392-pRK248/pTXI-1) (Lastick et al., 1986) that overproduces the enzyme by induction of the strong lambda PL promotor. Kinetic data, N-terminal sequence analysis, SDS polyacrylamide gel electrophoresis, size exclusion chromatography and immunodiffusion were used to compare the overproduced enzyme with xylose isomerase purified from xylose induced, non-transformed E. coli LE392 cells; no differences between these purified enzyme preparations were found.

Original languageAmerican English
Pages (from-to)79-84
Number of pages6
JournalBiotechnology Letters
Issue number2
StatePublished - 1988

NREL Publication Number

  • ACNR/JA-232-10212


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