Properties of Genetically Overproduced E. coli Xylose Isomerase

M. Y. Tucker; M. P. Tucker; M. E. Himmel; K. Grohmann; S. M. Lastick

doi:10.1007/BF01024630

Properties of Genetically Overproduced E. coli Xylose Isomerase

M. Y. Tucker, M. P. Tucker, M. E. Himmel, K. Grohmann, S. M. Lastick

BioEconomy and Sustainable Transportation

National Renewable Energy Laboratory

Research output: Contribution to journal › Article › peer-review

6 Scopus Citations

Abstract

Xylose isomerase was purified from a transformed E. coli strain (LE392-pRK248/pTXI-1) (Lastick et al., 1986) that overproduces the enzyme by induction of the strong lambda P_L promotor. Kinetic data, N-terminal sequence analysis, SDS polyacrylamide gel electrophoresis, size exclusion chromatography and immunodiffusion were used to compare the overproduced enzyme with xylose isomerase purified from xylose induced, non-transformed E. coli LE392 cells; no differences between these purified enzyme preparations were found.

Original language	American English
Pages (from-to)	79-84
Number of pages	6
Journal	Biotechnology Letters
Volume	10
Issue number	2
DOIs	https://doi.org/10.1007/BF01024630 https://doi.org/10.1007/BF01024630
State	Published - 1988

NREL Publication Number

ACNR/JA-232-10212

Access to Document

Cite this

@article{1144a664d9a743bea9720d65f3116125,

title = "Properties of Genetically Overproduced E. coli Xylose Isomerase",

abstract = "Xylose isomerase was purified from a transformed E. coli strain (LE392-pRK248/pTXI-1) (Lastick et al., 1986) that overproduces the enzyme by induction of the strong lambda PL promotor. Kinetic data, N-terminal sequence analysis, SDS polyacrylamide gel electrophoresis, size exclusion chromatography and immunodiffusion were used to compare the overproduced enzyme with xylose isomerase purified from xylose induced, non-transformed E. coli LE392 cells; no differences between these purified enzyme preparations were found.",

author = "Tucker, \{M. Y.\} and Tucker, \{M. P.\} and Himmel, \{M. E.\} and K. Grohmann and Lastick, \{S. M.\}",

year = "1988",

doi = "10.1007/BF01024630",

language = "American English",

volume = "10",

pages = "79--84",

journal = "Biotechnology Letters",

issn = "0141-5492",

publisher = "Springer Science and Business Media B.V.",

number = "2",

}

TY - JOUR

T1 - Properties of Genetically Overproduced E. coli Xylose Isomerase

AU - Tucker, M. Y.

AU - Tucker, M. P.

AU - Himmel, M. E.

AU - Grohmann, K.

AU - Lastick, S. M.

PY - 1988

Y1 - 1988

N2 - Xylose isomerase was purified from a transformed E. coli strain (LE392-pRK248/pTXI-1) (Lastick et al., 1986) that overproduces the enzyme by induction of the strong lambda PL promotor. Kinetic data, N-terminal sequence analysis, SDS polyacrylamide gel electrophoresis, size exclusion chromatography and immunodiffusion were used to compare the overproduced enzyme with xylose isomerase purified from xylose induced, non-transformed E. coli LE392 cells; no differences between these purified enzyme preparations were found.

AB - Xylose isomerase was purified from a transformed E. coli strain (LE392-pRK248/pTXI-1) (Lastick et al., 1986) that overproduces the enzyme by induction of the strong lambda PL promotor. Kinetic data, N-terminal sequence analysis, SDS polyacrylamide gel electrophoresis, size exclusion chromatography and immunodiffusion were used to compare the overproduced enzyme with xylose isomerase purified from xylose induced, non-transformed E. coli LE392 cells; no differences between these purified enzyme preparations were found.

UR - https://www.scopus.com/pages/publications/15844393734

U2 - 10.1007/BF01024630

DO - 10.1007/BF01024630

M3 - Article

AN - SCOPUS:15844393734

SN - 0141-5492

VL - 10

SP - 79

EP - 84

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 2

ER -

Properties of Genetically Overproduced E. coli Xylose Isomerase

Abstract

NREL Publication Number

Access to Document

Other files and links

Fingerprint

Cite this