Rapid Analysis of Carbohydrates in Bioprocess Samples: An Evaluation of the CarboPac SA10 for HPAE-PAD Analysis by Interlaboratory Comparison: Abstract No. AGFD-49

Research output: Contribution to conferencePaper

Abstract

A technique for carbohydrate analysis for bioprocess samples has been developed, providing reduced analysis time compared to current practice in the biofuels R&D community. The Thermofisher CarboPac SA10 anion-exchange column enables isocratic separation of monosaccharides, sucrose and cellobiose in approximately 7 minutes. Additionally, use of a low-volume (0.2 mL) injection valve in combinationwith a high-volume detection cell minimizes the extent of sample dilution required to bring sugar concentrations into the linear range of the pulsed amperometric detector (PAD). Three laboratories, representing academia, industry, and government, participated in an interlaboratory study which analyzed twenty-one opportunistic samples representing biomass pretreatment, enzymatic saccharification,and fermentation samples. The technique's robustness, linearity, and interlaboratory reproducibility were evaluated and showed excellent-to-acceptable characteristics. Additionally, quantitation by the CarboPac SA10/PAD was compared with the current practice method utilizing a HPX-87P/RID. While these two methods showed good agreement a statistical comparison found significant quantitationdifference between them, highlighting the difference between selective and universal detection modes.
Original languageAmerican English
StatePublished - 2013
EventAmerican Chemical Society. 245th ACS National Meeting - New Orleans, Louisiana
Duration: 7 Apr 201311 Apr 2013

Conference

ConferenceAmerican Chemical Society. 245th ACS National Meeting
CityNew Orleans, Louisiana
Period7/04/1311/04/13

NREL Publication Number

  • NREL/CP-5100-58870

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