Rapid Assessment of Lignin Content and Structure in Switchgrass (Panicum virgatum L.) Grown Under Different Environmental Conditions

David G.J. Mann, Nicole Labbé, Robert W. Sykes, Kristen Gracom, Lindsey Kline, Isabella M. Swamidoss, Jason N. Burris, Mark Davis, C. Neal Stewart

Research output: Contribution to journalArticlepeer-review

81 Scopus Citations

Abstract

Switchgrass (Panicum virgatum L.) is a candidate feedstock in bioenergy, and plant breeding and molecular genetic strategies are being used to improve germplasm. In order to assess these subsequent modifications, baseline biomass compositional data are needed in a relevant variety of environments. In this study, switchgrass cv. Alamo was grown in the field, greenhouse, and growth chamber and harvested into individual leaf and stem tissue components. These components were analyzed with pyrolysis vapor analysis using molecular beam mass spectrometry, Fourier transform infrared, and standard wet chemistry methods to characterize and compare the composition among the different growth environments. The details of lignin content, S/G ratios, and degree of cross-linked lignin are discussed. Multivariate approaches such as projection to latent structures regression found a very strong correlation between the lignin content obtained by standard wet chemistry methods and the two high throughput techniques employed to rapidly assess lignin in potential switchgrass candidates. The models were tested on unknown samples and verified by wet chemistry. The similar lignin content found by the two methods shows that both approaches are capable of determining lignin content in biomass in a matter of minutes.

Original languageAmerican English
Pages (from-to)246-256
Number of pages11
JournalBioenergy Research
Volume2
Issue number4
DOIs
StatePublished - 2009

NREL Publication Number

  • NREL/JA-510-47370

Keywords

  • Cell wall
  • FTIR
  • Lignin
  • PyMBMS
  • Recalcitrance
  • S/G ratio

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