Rapid Procedure for the Isolation and Purification of Photosynthetic Reaction Centers from Rhodopseudomonas sphaeroides R-26

Michael W. Kendall-Tobias, Michael Seibert

Research output: Contribution to journalArticlepeer-review

15 Scopus Citations

Abstract

A rapid purification procedure has been developed for the isolation of reaction centers From Rhodopseudomonas sphaeroides strain R-26. The procedure takes about 7 h and results in yields of 60-75%. The ratio of the optical absorbances at 280 and 800 nm is between 1.4 and 1.5, and preparations can be made with either one or two quinones per reaction center. EPR spectra show a sharp g 1.83 signal for the ubisemiquinone. The substitution of lauryl maltoside for lauryldimethylamine oxide suppresses reaction-center degradation in solution.

Original languageAmerican English
Pages (from-to)255-258
Number of pages4
JournalArchives of Biochemistry and Biophysics
Volume216
Issue number1
DOIs
StatePublished - 1982

NREL Publication Number

  • ACNR/JA-233-4928

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