Reply to Cosgrove: Non-Enzymatic Action of Expansins

Vivek Bharadwaj; Brandon Knott; Jerry Stahlberg; Gregg Beckham; Michael Crowley

doi:10.1074/jbc.RL120.013432

Reply to Cosgrove: Non-Enzymatic Action of Expansins

Vivek Bharadwaj, Brandon Knott, Jerry Stahlberg, Gregg Beckham, Michael Crowley

Swedish University of Agricultural Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

In our computational study, we use molecular simulations to substantiate a hypothetical mechanism for glycosidic bond cleavage in the presence of a single catalytic acid at the active site of the mutant D10N HiCel45A. In addition to discussing this plausible mechanism from the context of structurally related MltA lytic transglycosylase and subfamily C GH45s, we also suggest the implications of the plausible mechanism for our current understanding of the action of expansins and lytic transglycosylases.

Original language	American English
Pages (from-to)	6783
Number of pages	1
Journal	Journal of Biological Chemistry
Volume	295
Issue number	19
DOIs	https://doi.org/10.1074/jbc.RL120.013432 https://doi.org/10.1074/jbc.RL120.013432
State	Published - 2020

NREL Publication Number

NREL/JA-2800-77199

Keywords

expansins
molecular mechanisms

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abstract = "In our computational study, we use molecular simulations to substantiate a hypothetical mechanism for glycosidic bond cleavage in the presence of a single catalytic acid at the active site of the mutant D10N HiCel45A. In addition to discussing this plausible mechanism from the context of structurally related MltA lytic transglycosylase and subfamily C GH45s, we also suggest the implications of the plausible mechanism for our current understanding of the action of expansins and lytic transglycosylases.",

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AU - Stahlberg, Jerry

AU - Beckham, Gregg

AU - Crowley, Michael

PY - 2020

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AB - In our computational study, we use molecular simulations to substantiate a hypothetical mechanism for glycosidic bond cleavage in the presence of a single catalytic acid at the active site of the mutant D10N HiCel45A. In addition to discussing this plausible mechanism from the context of structurally related MltA lytic transglycosylase and subfamily C GH45s, we also suggest the implications of the plausible mechanism for our current understanding of the action of expansins and lytic transglycosylases.

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ER -

Reply to Cosgrove: Non-Enzymatic Action of Expansins

Abstract

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Keywords

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