The Role of Ester Groups in Resistance of Plant Cell Wall Polysaccharides to Enzymatic Hydrolysis

K. Grohmann; D. J. Mitchell; M. E. Himmel; B. E. Dale; H. A. Schroeder

doi:10.1007/BF02936472

The Role of Ester Groups in Resistance of Plant Cell Wall Polysaccharides to Enzymatic Hydrolysis

K. Grohmann, D. J. Mitchell, M. E. Himmel, B. E. Dale, H. A. Schroeder

Bioenergy Science and Technology

Research output: Contribution to journal › Article › peer-review

104 Scopus Citations

Abstract

Xylan backbones in native plant cell walls are extensively acety-lated. Previously, no direct investigations as to their role in cellulolytic enzyme resistance have been done, though indirect results point to their importance. An in vitro deesterification of aspen wood and wheat straw has been completed using hydroxylamine solutions. Yields of 90% acetyl ester removal for both materials have been accomplished, with little disruption of other fractions (i.e., lignin). Apparently, as the xylan becomes increasingly deacetylated, it becomes 5-7 times more digestible. This renders the cellulose fraction more accessible, and 2-3 times more digestible. This effect levels off near an acetyl removal of 75%, where other resistances become limiting.

Original language	American English
Pages (from-to)	45-61
Number of pages	17
Journal	Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume	20-21
Issue number	1
DOIs	https://doi.org/10.1007/BF02936472 https://doi.org/10.1007/BF02936472
State	Published - 1989

Bibliographical note

Work performed by the Solar Energy Research Institute, Golden, Colorado and Colorado State University, Fort Collins, Colorado

NREL Publication Number

ACNR/JA-230-11327

Keywords

cellulase
ester groups
pretreatment
Xylan

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abstract = "Xylan backbones in native plant cell walls are extensively acety-lated. Previously, no direct investigations as to their role in cellulolytic enzyme resistance have been done, though indirect results point to their importance. An in vitro deesterification of aspen wood and wheat straw has been completed using hydroxylamine solutions. Yields of 90% acetyl ester removal for both materials have been accomplished, with little disruption of other fractions (i.e., lignin). Apparently, as the xylan becomes increasingly deacetylated, it becomes 5-7 times more digestible. This renders the cellulose fraction more accessible, and 2-3 times more digestible. This effect levels off near an acetyl removal of 75%, where other resistances become limiting.",

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AU - Grohmann, K.

AU - Mitchell, D. J.

AU - Himmel, M. E.

AU - Dale, B. E.

AU - Schroeder, H. A.

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PY - 1989

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AB - Xylan backbones in native plant cell walls are extensively acety-lated. Previously, no direct investigations as to their role in cellulolytic enzyme resistance have been done, though indirect results point to their importance. An in vitro deesterification of aspen wood and wheat straw has been completed using hydroxylamine solutions. Yields of 90% acetyl ester removal for both materials have been accomplished, with little disruption of other fractions (i.e., lignin). Apparently, as the xylan becomes increasingly deacetylated, it becomes 5-7 times more digestible. This renders the cellulose fraction more accessible, and 2-3 times more digestible. This effect levels off near an acetyl removal of 75%, where other resistances become limiting.

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KW - pretreatment

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The Role of Ester Groups in Resistance of Plant Cell Wall Polysaccharides to Enzymatic Hydrolysis

Abstract

Bibliographical note

NREL Publication Number

Keywords

Access to Document

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