The Role of the Bidirectional Hydrogenase in Cyanobacteria

Damian Carrieri; Karen Wawrousek; Carrie Eckert; Jianping Yu; Pin Ching Maness

doi:10.1016/j.biortech.2011.03.103

The Role of the Bidirectional Hydrogenase in Cyanobacteria

Damian Carrieri, Karen Wawrousek, Carrie Eckert, Jianping Yu, Pin Ching Maness

National Renewable Energy Laboratory

Research output: Contribution to journal › Article › peer-review

76 Scopus Citations

Abstract

Cyanobacteria have tremendous potential to produce clean, renewable fuel in the form of hydrogen gas derived from solar energy and water. Of the two cyanobacterial enzymes capable of evolving hydrogen gas (nitrogenase and the bidirectional hydrogenase), the hox-encoded bidirectional Ni-Fe hydrogenase has a high theoretical potential. The physiological role of this hydrogenase is a highly debated topic and is poorly understood relative to that of the nitrogenase. Here the structure, assembly, and expression of this enzyme, as well as its probable roles in metabolism, are discussed and analyzed to gain perspective on its physiological role. It is concluded that the bidirectional hydrogenase in cyanobacteria primarily functions as a redox regulator for maintaining a proper oxidation/reduction state in the cell. Recommendations for future research to test this hypothesis are discussed.

Original language	American English
Pages (from-to)	8368-8377
Number of pages	10
Journal	Bioresource Technology
Volume	102
Issue number	18
DOIs	https://doi.org/10.1016/j.biortech.2011.03.103 https://doi.org/10.1016/j.biortech.2011.03.103
State	Published - 2011

NREL Publication Number

NREL/JA-2700-51293

Keywords

Bidirectional hydrogenase
Cyanobacteria
Hox hydrogenase
Hydrogen
Hydrogenase

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title = "The Role of the Bidirectional Hydrogenase in Cyanobacteria",

abstract = "Cyanobacteria have tremendous potential to produce clean, renewable fuel in the form of hydrogen gas derived from solar energy and water. Of the two cyanobacterial enzymes capable of evolving hydrogen gas (nitrogenase and the bidirectional hydrogenase), the hox-encoded bidirectional Ni-Fe hydrogenase has a high theoretical potential. The physiological role of this hydrogenase is a highly debated topic and is poorly understood relative to that of the nitrogenase. Here the structure, assembly, and expression of this enzyme, as well as its probable roles in metabolism, are discussed and analyzed to gain perspective on its physiological role. It is concluded that the bidirectional hydrogenase in cyanobacteria primarily functions as a redox regulator for maintaining a proper oxidation/reduction state in the cell. Recommendations for future research to test this hypothesis are discussed.",

keywords = "Bidirectional hydrogenase, Cyanobacteria, Hox hydrogenase, Hydrogen, Hydrogenase",

author = "Damian Carrieri and Karen Wawrousek and Carrie Eckert and Jianping Yu and Maness, {Pin Ching}",

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T1 - The Role of the Bidirectional Hydrogenase in Cyanobacteria

AU - Carrieri, Damian

AU - Wawrousek, Karen

AU - Eckert, Carrie

AU - Yu, Jianping

AU - Maness, Pin Ching

PY - 2011

Y1 - 2011

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AB - Cyanobacteria have tremendous potential to produce clean, renewable fuel in the form of hydrogen gas derived from solar energy and water. Of the two cyanobacterial enzymes capable of evolving hydrogen gas (nitrogenase and the bidirectional hydrogenase), the hox-encoded bidirectional Ni-Fe hydrogenase has a high theoretical potential. The physiological role of this hydrogenase is a highly debated topic and is poorly understood relative to that of the nitrogenase. Here the structure, assembly, and expression of this enzyme, as well as its probable roles in metabolism, are discussed and analyzed to gain perspective on its physiological role. It is concluded that the bidirectional hydrogenase in cyanobacteria primarily functions as a redox regulator for maintaining a proper oxidation/reduction state in the cell. Recommendations for future research to test this hypothesis are discussed.

KW - Bidirectional hydrogenase

KW - Cyanobacteria

KW - Hox hydrogenase

KW - Hydrogen

KW - Hydrogenase

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The Role of the Bidirectional Hydrogenase in Cyanobacteria

Abstract

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Keywords

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