Selective Synthesis of Human Milk Fat-Style Structured Triglycerides from Microalgal Oil in a Microfluidic Reactor Packed with Immobilized Lipase

Tao Dong, Jun Wang, Xi Liu, Xu-Dong Wang, Xing-Yu Zhao, Dan Zhu, Yi-Yuan Mei, Guo-Hua Wu

Research output: Contribution to journalArticlepeer-review

38 Scopus Citations

Abstract

Human milk fat-style structured triacylglycerols were produced from microalgal oil in a continuous microfluidic reactor packed with immobilized lipase for the first time. A remarkably high conversion efficiency was demonstrated in the microreactor with reaction time being reduced by 8 times, Michaelis constant decreased 10 times, the lipase reuse times increased 2.25-fold compared to those in a batch reactor. In addition, the content of palmitic acid at sn-2 position (89.0%) and polyunsaturated fatty acids at sn-1, 3 positions (81.3%) are slightly improved compared to the product in a batch reactor. The increase of melting points (1.7 °C) and decrease of crystallizing point (3 °C) implied higher quality product was produced using the microfluidic technology. The main cost can be reduced from $212.3 to $14.6 per batch with the microreactor. Overall, the microfluidic bioconversion technology is promising for modified functional lipids production allowing for cost-effective approach to produce high-value microalgal coproducts.

Original languageAmerican English
Pages (from-to)132-141
Number of pages10
JournalBioresource Technology
Volume220
DOIs
StatePublished - 1 Nov 2016

Bibliographical note

Publisher Copyright:
© 2016 Elsevier Ltd

NREL Publication Number

  • NREL/JA-5100-67110

Keywords

  • Coproduct
  • Microalgae oil
  • Microfluidic bioconversion
  • Polyunsaturated fatty acid
  • Structured triacylglycerols

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