Simultaneous Saccharification and Cofermentation of Dilute-Acid Pretreated Yellow Poplar Hardwood to Ethanol Using Xylose-Fermenting Zymomonas mobilis

James D. McMillan, Mildred M. Newman, David W. Templeton, Ali Mohagheghi

Research output: Contribution to journalArticlepeer-review

87 Scopus Citations

Abstract

Simultaneous saccharification and cofermentation (SSCF) was carried out at approximately 15% total solids using conditioned dilute-acid pretreated yellow poplar feedstock, an adapted variant of National Renewable Energy Laboratory (NREL) xylose-fermenting Zymomonas mobilis and either commercial or NREL-produced cellulase enzyme preparations. In 7 d, at a cellulase loading of 12 filter paper units per gram cellulose (FPU/g), the integrated system produced more than 3% w/v ethanol and achieved 54% conversion of all potentially available biomass sugars (total sugars) entering SSCF. A control SSCF employing Sigmacell cellulose and a commercial cellulase at an enzyme loading of 14 FPU/g achieved 65% conversion of total sugars to ethanol.

Original languageAmerican English
Pages (from-to)649-665
Number of pages17
JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume77-79
DOIs
StatePublished - 1999

NREL Publication Number

  • NREL/JA-580-24686

Keywords

  • Enzymatic saccharification
  • Ethanol fermentation
  • Hydrolyzate conditioning
  • Integrated testing
  • SSCF
  • Zymomonas mobilis

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