Abstract
Simultaneous saccharification and cofermentation (SSCF) was carried out at approximately 15% total solids using conditioned dilute-acid pretreated yellow poplar feedstock, an adapted variant of National Renewable Energy Laboratory (NREL) xylose-fermenting Zymomonas mobilis and either commercial or NREL-produced cellulase enzyme preparations. In 7 d, at a cellulase loading of 12 filter paper units per gram cellulose (FPU/g), the integrated system produced more than 3% w/v ethanol and achieved 54% conversion of all potentially available biomass sugars (total sugars) entering SSCF. A control SSCF employing Sigmacell cellulose and a commercial cellulase at an enzyme loading of 14 FPU/g achieved 65% conversion of total sugars to ethanol.
Original language | American English |
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Pages (from-to) | 649-665 |
Number of pages | 17 |
Journal | Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology |
Volume | 77-79 |
DOIs | |
State | Published - 1999 |
NREL Publication Number
- NREL/JA-580-24686
Keywords
- Enzymatic saccharification
- Ethanol fermentation
- Hydrolyzate conditioning
- Integrated testing
- SSCF
- Zymomonas mobilis