Spectral Deconvolution of Electron-Bifurcating Flavoproteins

Steve Ortiz; Dimitri Niks; Wayne Vigil Jr.; Jessica Tran; Carolyn Lubner; Russ Hille

doi:10.1016/bs.mie.2023.03.011

Spectral Deconvolution of Electron-Bifurcating Flavoproteins

Steve Ortiz, Dimitri Niks, Wayne Vigil Jr., Jessica Tran, Carolyn Lubner, Russ Hille

Biosciences Center

University of California, Riverside

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

1 Scopus Citations

Abstract

Electron-bifurcating flavoproteins catalyze the tightly coupled reduction of high- and low-potential acceptors using a median-potential electron donor, and are invariably complex systems with multiple redox-active centers in two or more subunits. Methods are described that permit, in favorable cases, the deconvolution of spectral changes associated with reduction of specific centers, making it possible to dissect the overall process of electron bifurcation into individual, discrete steps.

Original language	American English
Title of host publication	New Experimental Probes for Enzyme Specificity and Mechanism
Editors	John P. Richard, Graham R. Moran
Publisher	Academic Press Inc.
Pages	531-550
Number of pages	20
ISBN (Print)	9780443152764
DOIs	https://doi.org/10.1016/bs.mie.2023.03.011 https://doi.org/10.1016/bs.mie.2023.03.011
State	Published - 2023

Publication series

Name	Methods in Enzymology
Volume	685
ISSN (Print)	0076-6879
ISSN (Electronic)	1557-7988

Bibliographical note

Publisher Copyright:
© 2023 Elsevier Inc.

NREL Publication Number

NREL/CH-2700-86258

Keywords

Electron bifurcation
Electron paramagnetic resonance
Flavoproteins
Transient absorbance spectroscopy

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Ortiz, S., Niks, D., Vigil Jr., W., Tran, J., Lubner, C., & Hille, R. (2023). Spectral Deconvolution of Electron-Bifurcating Flavoproteins. In J. P. Richard, & G. R. Moran (Eds.), New Experimental Probes for Enzyme Specificity and Mechanism (pp. 531-550). (Methods in Enzymology; Vol. 685). Academic Press Inc.. https://doi.org/10.1016/bs.mie.2023.03.011, https://doi.org/10.1016/bs.mie.2023.03.011

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abstract = "Electron-bifurcating flavoproteins catalyze the tightly coupled reduction of high- and low-potential acceptors using a median-potential electron donor, and are invariably complex systems with multiple redox-active centers in two or more subunits. Methods are described that permit, in favorable cases, the deconvolution of spectral changes associated with reduction of specific centers, making it possible to dissect the overall process of electron bifurcation into individual, discrete steps.",

keywords = "Electron bifurcation, Electron paramagnetic resonance, Flavoproteins, Transient absorbance spectroscopy",

author = "Steve Ortiz and Dimitri Niks and {Vigil Jr.}, Wayne and Jessica Tran and Carolyn Lubner and Russ Hille",

note = "Publisher Copyright: {\textcopyright} 2023 Elsevier Inc.",

year = "2023",

doi = "10.1016/bs.mie.2023.03.011",

language = "American English",

isbn = "9780443152764",

series = "Methods in Enzymology",

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Ortiz, S, Niks, D, Vigil Jr., W, Tran, J, Lubner, C & Hille, R 2023, Spectral Deconvolution of Electron-Bifurcating Flavoproteins. in JP Richard & GR Moran (eds), New Experimental Probes for Enzyme Specificity and Mechanism. Methods in Enzymology, vol. 685, Academic Press Inc., pp. 531-550. https://doi.org/10.1016/bs.mie.2023.03.011, https://doi.org/10.1016/bs.mie.2023.03.011

Spectral Deconvolution of Electron-Bifurcating Flavoproteins. / Ortiz, Steve; Niks, Dimitri; Vigil Jr., Wayne et al.
New Experimental Probes for Enzyme Specificity and Mechanism. ed. / John P. Richard; Graham R. Moran. Academic Press Inc., 2023. p. 531-550 (Methods in Enzymology; Vol. 685).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

TY - CHAP

T1 - Spectral Deconvolution of Electron-Bifurcating Flavoproteins

AU - Ortiz, Steve

AU - Niks, Dimitri

AU - Vigil Jr., Wayne

AU - Tran, Jessica

AU - Lubner, Carolyn

AU - Hille, Russ

PY - 2023

Y1 - 2023

N2 - Electron-bifurcating flavoproteins catalyze the tightly coupled reduction of high- and low-potential acceptors using a median-potential electron donor, and are invariably complex systems with multiple redox-active centers in two or more subunits. Methods are described that permit, in favorable cases, the deconvolution of spectral changes associated with reduction of specific centers, making it possible to dissect the overall process of electron bifurcation into individual, discrete steps.

AB - Electron-bifurcating flavoproteins catalyze the tightly coupled reduction of high- and low-potential acceptors using a median-potential electron donor, and are invariably complex systems with multiple redox-active centers in two or more subunits. Methods are described that permit, in favorable cases, the deconvolution of spectral changes associated with reduction of specific centers, making it possible to dissect the overall process of electron bifurcation into individual, discrete steps.

KW - Electron bifurcation

KW - Electron paramagnetic resonance

KW - Flavoproteins

KW - Transient absorbance spectroscopy

UR - http://www.scopus.com/inward/record.url?scp=85153118516&partnerID=8YFLogxK

U2 - 10.1016/bs.mie.2023.03.011

DO - 10.1016/bs.mie.2023.03.011

M3 - Chapter

C2 - 37245914

AN - SCOPUS:85153118516

SN - 9780443152764

T3 - Methods in Enzymology

SP - 531

EP - 550

BT - New Experimental Probes for Enzyme Specificity and Mechanism

A2 - Richard, John P.

A2 - Moran, Graham R.

PB - Academic Press Inc.

ER -

Spectral Deconvolution of Electron-Bifurcating Flavoproteins

Abstract

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Keywords

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