Spectral Deconvolution of Electron-Bifurcating Flavoproteins

Steve Ortiz, Dimitri Niks, Wayne Vigil Jr., Jessica Tran, Carolyn Lubner, Russ Hille

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Electron-bifurcating flavoproteins catalyze the tightly coupled reduction of high- and low-potential acceptors using a median-potential electron donor, and are invariably complex systems with multiple redox-active centers in two or more subunits. Methods are described that permit, in favorable cases, the deconvolution of spectral changes associated with reduction of specific centers, making it possible to dissect the overall process of electron bifurcation into individual, discrete steps.

Original languageAmerican English
Title of host publicationNew Experimental Probes for Enzyme Specificity and Mechanism
EditorsJohn P. Richard, Graham R. Moran
PublisherAcademic Press Inc.
Pages531-550
Number of pages20
ISBN (Print)9780443152764
DOIs
StatePublished - 2023

Publication series

NameMethods in Enzymology
Volume685
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Bibliographical note

Publisher Copyright:
© 2023 Elsevier Inc.

NREL Publication Number

  • NREL/CH-2700-86258

Keywords

  • Electron bifurcation
  • Electron paramagnetic resonance
  • Flavoproteins
  • Transient absorbance spectroscopy

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