Spectral, Photophysical, and Stability Properties of Isolated Photosystem II Reaction Center

Michael Seibert, Rafael Picorel, Andrew B. Rubin, John S. Connolly

Research output: Contribution to journalArticlepeer-review

127 Scopus Citations


Photosystem II reaction center (RC) preparations isolated from spinach (Spinacea olerácea) by the Nanba-Satoh procedure (O Nanba,K Satoh 1987 Proc Natl Acad Sci USA 84: 109-112) are quite labile, even at 4°C in the dark. Simple spectroscopic criteria were developed to characterize the native state of the material. Degradation of the RC results in (a) blue-shifting of the red-most absorption maximum, (b) a shift of the 77K fluorescence maximum from ~682 nm to ~670 nm, and (c) a shift of fluorescence lifetime components from 1.3-4 nanoseconds and >25 nanoseconds to~6-7 nanoseconds. Fluorescence properties at 77K seem to be a more sensitive spectral indicator of the integrity of the material. The >25 nanosecond lifetime component is assigned to P680+ Pheophytin-recombination luminescence, which suggests a correlation between the observed spectral shifts and the photochemical competence of the preparation. Substitution of lauryl maltoside for Triton X-100 immediately after RC isolation stabilizes the RCs and suggests that Triton may be responsible for the instability.

Original languageAmerican English
Pages (from-to)303-306
Number of pages4
JournalPlant Physiology
Issue number2
StatePublished - 1988

Bibliographical note

Publisher Copyright:
© 1988 American Society of Plant Biologists. All rights reserved.

NREL Publication Number

  • SERI/JA-21147


Dive into the research topics of 'Spectral, Photophysical, and Stability Properties of Isolated Photosystem II Reaction Center'. Together they form a unique fingerprint.

Cite this