Spectral Properties of Stabilized D1/D2/Cytochrome b-559 Photosystem II Reaction Center Complex: Effects of Triton X-100, the Redox State of Pheophytin and B-Carotene

V. L. Tetenkin; B. A. Gulyaev; M. Seibert; A. B. Rubin

doi:10.1016/0014-5793(89)80776-8

Spectral Properties of Stabilized D1/D2/Cytochrome b-559 Photosystem II Reaction Center Complex: Effects of Triton X-100, the Redox State of Pheophytin and B-Carotene

V. L. Tetenkin, B. A. Gulyaev, M. Seibert, A. B. Rubin

BioEconomy and Sustainable Transportation

Lomonosov Moscow State University

Research output: Contribution to journal › Article › peer-review

92 Scopus Citations

Abstract

Absorption, fluorescence, and CD spectral properties of the isolated D1/D2/cytochrome b-559 photosystem II reaction center complex were examined in stabilized reaction center material at 77 K. Spectral properties were dependent on the presence or absence of 0.05% Triton X-100 in the RC suspension medium, on the redox state of pheophytin, and on the state of inactivation of the complex. The specific spectral properties of the PS II RC complex in the red suggest that the primary donor is not a bacterial-type special pair and could be a monomer. Furthermore, the spectral properties in the PS II RC may be the result of excitonic interactions among all the porphyrin molecules in the complex. Interactions between β-carotene and porphyrins indicate a significant role for β-carotene in the PS II RC.

Original language	American English
Pages (from-to)	459-463
Number of pages	5
Journal	FEBS Letters
Volume	250
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(89)80776-8 https://doi.org/10.1016/0014-5793(89)80776-8
State	Published - 1989

Bibliographical note

Work performed by Moscow Lomonosov State University, Moscow, Union of Soviet Socialist Republics, and Solar Energy Research Institute, Golden, Colorado

NREL Publication Number

ACNR/JA-233-11350

Keywords

(Spinach)
Carotene, β-
Detergent effect
Photosynthesis
Photosystem II
Reaction center
Spectroscopy

Access to Document

Cite this

@article{fc234ac5f08e4f37b0650fea9d37dfeb,

title = "Spectral Properties of Stabilized D1/D2/Cytochrome b-559 Photosystem II Reaction Center Complex: Effects of Triton X-100, the Redox State of Pheophytin and B-Carotene",

abstract = "Absorption, fluorescence, and CD spectral properties of the isolated D1/D2/cytochrome b-559 photosystem II reaction center complex were examined in stabilized reaction center material at 77 K. Spectral properties were dependent on the presence or absence of 0.05% Triton X-100 in the RC suspension medium, on the redox state of pheophytin, and on the state of inactivation of the complex. The specific spectral properties of the PS II RC complex in the red suggest that the primary donor is not a bacterial-type special pair and could be a monomer. Furthermore, the spectral properties in the PS II RC may be the result of excitonic interactions among all the porphyrin molecules in the complex. Interactions between β-carotene and porphyrins indicate a significant role for β-carotene in the PS II RC.",

keywords = "(Spinach), Carotene, β-, Detergent effect, Photosynthesis, Photosystem II, Reaction center, Spectroscopy",

author = "Tetenkin, {V. L.} and Gulyaev, {B. A.} and M. Seibert and Rubin, {A. B.}",

note = "Work performed by Moscow Lomonosov State University, Moscow, Union of Soviet Socialist Republics, and Solar Energy Research Institute, Golden, Colorado",

year = "1989",

doi = "10.1016/0014-5793(89)80776-8",

language = "American English",

volume = "250",

pages = "459--463",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "2",

}

TY - JOUR

T1 - Spectral Properties of Stabilized D1/D2/Cytochrome b-559 Photosystem II Reaction Center Complex: Effects of Triton X-100, the Redox State of Pheophytin and B-Carotene

AU - Tetenkin, V. L.

AU - Gulyaev, B. A.

AU - Seibert, M.

AU - Rubin, A. B.

N1 - Work performed by Moscow Lomonosov State University, Moscow, Union of Soviet Socialist Republics, and Solar Energy Research Institute, Golden, Colorado

PY - 1989

Y1 - 1989

N2 - Absorption, fluorescence, and CD spectral properties of the isolated D1/D2/cytochrome b-559 photosystem II reaction center complex were examined in stabilized reaction center material at 77 K. Spectral properties were dependent on the presence or absence of 0.05% Triton X-100 in the RC suspension medium, on the redox state of pheophytin, and on the state of inactivation of the complex. The specific spectral properties of the PS II RC complex in the red suggest that the primary donor is not a bacterial-type special pair and could be a monomer. Furthermore, the spectral properties in the PS II RC may be the result of excitonic interactions among all the porphyrin molecules in the complex. Interactions between β-carotene and porphyrins indicate a significant role for β-carotene in the PS II RC.

AB - Absorption, fluorescence, and CD spectral properties of the isolated D1/D2/cytochrome b-559 photosystem II reaction center complex were examined in stabilized reaction center material at 77 K. Spectral properties were dependent on the presence or absence of 0.05% Triton X-100 in the RC suspension medium, on the redox state of pheophytin, and on the state of inactivation of the complex. The specific spectral properties of the PS II RC complex in the red suggest that the primary donor is not a bacterial-type special pair and could be a monomer. Furthermore, the spectral properties in the PS II RC may be the result of excitonic interactions among all the porphyrin molecules in the complex. Interactions between β-carotene and porphyrins indicate a significant role for β-carotene in the PS II RC.

KW - (Spinach)

KW - Carotene, β-

KW - Detergent effect

KW - Photosynthesis

KW - Photosystem II

KW - Reaction center

KW - Spectroscopy

UR - http://www.scopus.com/inward/record.url?scp=0000087299&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(89)80776-8

DO - 10.1016/0014-5793(89)80776-8

M3 - Article

AN - SCOPUS:0000087299

SN - 0014-5793

VL - 250

SP - 459

EP - 463

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Spectral Properties of Stabilized D1/D2/Cytochrome b-559 Photosystem II Reaction Center Complex: Effects of Triton X-100, the Redox State of Pheophytin and B-Carotene

Abstract

Bibliographical note

NREL Publication Number

Keywords

Access to Document

Other files and links

Fingerprint

Cite this