Squeezed Light Stimulated Raman Scattering (SRS) Microscopy for Bioenergy Applications

Yining Zeng, Zhenhuan Yi, Girish Agarwal, Marian Scully, Bryon Donohoe

Research output: NRELPresentation


Stimulated Raman scattering (SRS) microscopy using picosecond near-IR pulses have provided a great penetration depth with reduced fluorescence interference when imaging biological samples for bioenergy applications. These tools have provided insight into 1) tracking the degradation of chemical composites in biomass feedstocks to investigate the recalcitrant factors during the deconstruction processes, 2) monitoring the production of chemicals in photosynthetic plants and wood-digesting microorganisms, and 3) probing plant-bacteria interactions. However, the above processes are usually slow and require continuous imaging for an extended period. This is challenging for classic SRS because the laser power needed to achieve enough sensitivity causes photodamage in the samples during such long experiments. Quantum-squeezed light with reduced noise in the intensity quadrature can improve the sensitivity of classic SRS microscopy beyond the shot noise limit. The successful squeezing of one of the picosecond pulses in the above SRS will improve sensitivity and reduce photodamage, greatly expanding the range of studies available to SRS microscopy.
Original languageAmerican English
Number of pages17
StatePublished - 2024

Publication series

NamePresented at the Advanced Chemical Microscopy for Life Science and Translational Medicine Conference, 27-29 January 2024, San Francisco, California

Bibliographical note

Video of presentation posted on SPIE Digital Library

NREL Publication Number

  • NREL/PR-2800-87842


  • quantum
  • squeezed light
  • stimulated Raman scattering microscopy


Dive into the research topics of 'Squeezed Light Stimulated Raman Scattering (SRS) Microscopy for Bioenergy Applications'. Together they form a unique fingerprint.

Cite this